膀胱癌患者癌组织ABCC4、miR506-3-p表达水平及临床意义

Expression levels of ABCC4 and miR-506-3p in cancer tissues of patients with bladder cancer and their clinical significance

目的检测膀胱癌患者ATP结合盒转运蛋白C4(ABCC4)和微小RNA-506-3p(miR-506-3p)的表达水平,并探讨其与患者预后的关系。方法选取2015年5月至2017年5月该院收治的78例膀胱癌患者作为研究对象,术中收集所有患者膀胱癌组织及癌旁组织(距离癌组织边缘≥3 cm)。采用免疫印迹法或实时荧光定量PCR技术检测各组织ABCC4蛋白、mRNA及miR-506-3p的表达水平,分析ABCC4蛋白和miR-506-3p表达的相关性、靶向调控关系,以及二者与膀胱癌患者临床病理特征、术后4年总生存率的关系;COX回归分析影响膀胱癌患者预后的危险因素。结果膀胱癌组织ABCC4蛋白和mRNA的表达水平均明显高于癌旁组织(P<0.05),而膀胱癌组织miR-506-3p的表达水平明显低于癌旁组织(P<0.05)。膀胱癌组织中ABCC4蛋白与miR-506-3p表达呈负相关(P<0.05),且miR-506-3p可靶向调控ABCC4;膀胱癌组织ABCC4蛋白的表达水平与肿瘤分期、肿瘤最大径和是否发生淋巴结转移有关(P<0.05),miR-506-3p表达水平与肿瘤分期有关(P<0.05)。ABCC4蛋白高表达组患者总生存率明显低于低表达组(P<0.05),miR-506-5p高表达组总生存率明显高于低表达组(P<0.05);肿瘤分期及膀胱癌组织ABCC4蛋白、miR-506-3p表达是影响膀胱癌患者预后的独立危险因素(P<0.05)。结论ABCC4在膀胱癌组织中表达上调,而miR-506-3p在膀胱癌组织中表达下调,且二者呈负相关。ABCC4、miR-506-3p均具有作为膀胱癌预后标志物和治疗靶点的潜力。

Objective To detect the expression levels of ATP-binding cassette transporter C4(ABCC4)and microRNA-506-3p(miR-506-3p)in patients with bladder cancer and to investigate their relationship with prognosis.Methods Seventy-eight patients with bladder cancer admitted to this hospital from May 2015 to May 2017 were selected as the research objects.The bladder cancer tissues and paracancer tissues(≥3 cm from the margin of cancer tissue)of all patients were collected during the operation.The relative expression of ABCC4 protein,ABCC4 mRNA and miR-506-3p in each tissues was detected by Western blotting or fluorescence quantitative PCR.The correlation and targeted regulatory relationship between the expression of ABCC4 and miR-506-3p and the relationship between the expression of ABCC4 protein and miR-506-3p were analyzed,as well as their relationship with clinicopathological features and 4-year overall survival rate of patients with bladder cancer.COX regression was used to analyze the risk factors affecting the prognosis of patients with bladder cancer.Results The expression levels of ABCC4 protein and mRNA in bladder cancer tissues were significantly higher than those in paracancer tissues(P<0.05),while the expression level of miR-506-3p in bladder cancer tissues was significantly lower than that in paracancer tissues(P<0.05).ABCC4 protein was negatively correlated with the expression of miR-506-3p in bladder cancer tissues(P<0.05),and ABCC4 regulation was targeted by miR-506-3p.The protein expression level of ABCC4 in bladder cancer tissues was correlated with tumor stage,maximum tumor diameter and lymph node metastasis(P<0.05),and the expression of miR-506-3p was related to tumor stage(P<0.05).The overall survival rate of patients in the ABCC4 high expression group was significantly lower than that in the low expression group(P<0.05),and the overall survival rate in miR-506-5p high expression group was significantly higher than that in the low expression group(P<0.05).Tumor stage and the expression of ABCC4 protein and miR-506-3p in bladder cancer were independent risk factors affecting the prognosis of patients with bladder cancer(P<0.05).Conclusion ABCC4 is up-regulated in bladder cancer tissues,while miR-506-3p is down-regulated in bladder cancer tissues,and the two are negatively correlated.Both ABCC4 and miR-506-3p have the potential to serve as prognostic markers and therapeutic targets for bladder cancer.