摘要
为明确甘肃省民乐县采集的藜霜霉病病原菌的种类,文章对其进行常规形态学鉴定,提取了菌物的DNA,利用cox1、cox2-mtDNA和ITS rDNA基因序列进行分子鉴定,并构建了系统发育树,以明确其分类地位。形态学鉴定结果和基于cox1、cox2、ITS序列的系统发育树均表明该病原菌为藜麦霜霉菌Peronospora variabilis。
In order to identify the pathogen species of downy mildew of Chenopodium album collected in Minle County of Gansu Province,this paper carried out routine morphological identification,extracted fungal DNA,and identified the pathogen using cox1,cox2 mtDNA and ITS rDNA gene sequences,and constructed a phylogenetic tree to clarify its taxonomic position.The morphological identification results and phylogenetic trees based on cox1,cox2,and ITS sequences all indicate that the pathogen is Peronospora variabilis.
作者
高国香
王艳
张婷
GAO Guoxiang;WANG Yan;ZHANG Ting(Gansu University of Traditional Chinese Medicine School of Pharmacy,Lanzhou,730000,China;Northwest Chinese Tibetan Medicine Collaborative Innovation Center,Lanzhou 730000,China)
出处
《工业微生物》
CAS
2023年第5期119-121,共3页
Industrial Microbiology
基金
国家自然科学基金(31960004)
中央本级重大增减支项目“名贵中药资源可持续利用能力建设”(2060302)
西北中藏药省部共建协同创新中心开放基金项目(Xbzzy-2022-02)
道地药材生态种植及质量保障项目(国中医药科技[2020]153号)
甘肃省2021年度优秀研究生“创新之星”(2021CXZX753)。
关键词
藜
霜霉病
病原鉴定
藜麦霜霉菌
Chenopodiumalbum
downy mildews
pathogen identify
Peronospora variabilis
