CSIG、SIRT1、CYR61、IL-8表达与良性前列腺增生临床进展相关性分析

Correlation analysis between the expression of CSIG,SIRT1,CYR61,IL-8 and the clinical progression of benign prostatic hyperplasia

目的:很多分子标记物的表达异常与良性前列腺增生(benign prostatic hyperplasia,BPH)临床进展密切相关,本研究探讨BPH组织中细胞衰老抑制基因(CSIG)、沉默信息调节因子2相关酶1(SIRT1)、富半胱氨酸61(CYR61)和白细胞介素-8(IL-8)蛋白表达水平,并分析与BPH临床进展相关因素之间相关性。方法:收集96例接受BPH手术患者的前列腺组织作为临床进展组,并且收集20例行全膀胱手术且无明显下尿路症状患者的前列腺组织作为对照组。临床数据如年龄、前列腺体积(PV)、血清前列腺特异性抗原(PSA)水平、国际前列腺症状评分(IPSS)、体重指数(BMI)、血糖(GLU)、甘油三酯(TG)、总胆固醇(TC)、高密度脂蛋白(HDL)、低密度脂蛋白(LDL)进行记录,利用Western-blot检测前列腺组织中CSIG、SIRT1、CYR61和IL-8蛋白表达水平,并与上述BPH临床数据进行相关性分析,免疫组化ABC法对前列腺组织中蛋白表达定位进行检测。结果:Western-blot结果表明与对照组比较,BPH临床进展组中CSIG、SIRT1、CYR61和IL-8蛋白表达水平显著增高(P<0.05),免疫组化结果显示BPH临床进展组中CSIG、SIRT1、CYR61和IL-8阳性表达率分别为58.3%(56/96)、41.7%(40/96)、75.0%(72/96)和52.1%(50/96)。CSIG和CYR61蛋白表达水平与患者年龄、PV、血清PSA、TG呈正相关(P<0.05),SIRT1蛋白表达水平与患者年龄、BMI呈正相关(P<0.05),IL-8蛋白表达水平与患者年龄、PV、血清PSA呈正相关(P<0.05)。结论:CSIG、SIRT1、CYR61和IL-8表达异常与BPH临床进展危险因素均存在不同程度的相关性,CSIG对BPH临床进展促进作用最为显著。

Objective:To investigate the expression of cellular senescence inhibited gene(CSIG),silent mat-ing-type information regulation 2 homolog 1(SIRT1),CYR61 and interleukin-8(IL-8)in BPH tissue specimens,and to analyze the correlation between these biomarkers and the clinical progression of BPH.Methods:All tissue specimens from 96 BPH cases who underwent transurethral resection of the prostate(TURP)were processed,and the prostate tissues of 20 patients without lower urinary tract symptoms and undergoing radical bladder sur-gery were collected as the control group.Patient age,prostate volume,serum prostate specific antigen(PSA)lev-el,international prostate symptom score(IPSS),body mass index(BMI),glucose(GLU),triglyceride(TG),total cholesterol(TC),high-density lipoprotein(HDL),low-density lipoprotein(LDL)of BPH cases were col-lected before surgery.The expressions of CSIG,SIRT1,CYR6l and IL-8 were examined by Western-blot and im-munohistochemistry(IHC)in BPH specimens,and possible association between these biomarkers and the data of BPH was also analyzed.Results:Compared with control group,the expressions of CSIG,SIRT1,CYR61 and IL-8 were significantly higher in BPH clinical progression group(P<0.05).IHC results indicated that the positive rates of CSIG,SIRT1,CYR61 and IL-8 were 58.3%(56/96),41.7%(40/96),75.0%(72/96)and 52.1%(50/96),respectively.The expressions of both CSIG and CYR61 were positively correlated with patient age,prostate volume,serum PSA level and TG(P<0.05).Furthermore,SIRT1 expression was positively related to age and BMI(P<0.05),and IL-8 expression was positively related to patient age,prostate volume,serum PSA level(P<0.05).Conclusion:The higher expressions of CSIG,SIRT1,CYR61 and IL-8 are related to the risk factors of BPH clinical progression,and CSIG plays the most significant role in promoting BPH clinical progression.