L-乳酸促进皮下脂肪细胞棕脂分化和产热

L-lactate promotes subcutaneous adipocyte browning and thermogenesis

文章为模拟适度运动后的乳酸水平,用5 mmol/L和10 mmol/L的L-乳酸钠处理原代培养的皮下脂肪基质血管组分细胞和分化后的棕色脂肪细胞,检测产热基因、UCP1蛋白和AMPK磷酸化蛋白表达水平,结果表明,L-乳酸处理促进了皮下脂肪细胞分化过程中和分化成熟后产热基因和UCP1蛋白的表达。AMPK信号参与了L-乳酸促进棕脂细胞产热的进程。研究结果为乳酸改善机体代谢提供新的理论依据,并为肥胖及其相关的代谢性疾病提供了一种新的预防和治疗手段。

In order to simulate the moderate exercise derived lactate level,the primary subcutaneous adipose stromal vascular fraction cells and differentiated brown adipocytes were treated with 5 mmol/L and 10 mmol/L L-lactate sodium.The expression of thermogenic genes,UCP1 protein and AMPK phosphorylated protein were detected.The results showed that L-lactate treatment promoted the expression of thermogenic genes and UCP1 protein during and after brown differentiation of subcutaneous adipocytes.AMPK signaling was involved in the promotion of L-lactate on brown adipocyte thermogenesis.These results provide new theoretical basis for lactate to improve body metabolism,and provide a new approach for the prevention and treatment of obesity and its related metabolic diseases.