急性减压缺氧对大鼠血液学指标的影响

Effects of Hematological Parameters by Acute Decompression Hypoxia in Rats

目的探讨模拟急性高原低压性缺氧对大鼠血液指标变化的影响。方法建立高原缺氧大鼠损伤模型,将40只Wistar雄性大鼠随机分为常氧对照组、缺氧10 h组、缺氧20 h组、缺氧30 h组,每组10只,对照组置于当地海拔1500 m处,缺氧10、20、30 h组放入大型低压低氧动物实验舱,模拟海拔8000 m高原低压性缺氧环境,检测大鼠血气、血常规和血生化指标,观察不同缺氧时间对大鼠血液学指标的影响。结果随着缺氧时间的延长,与常氧对照组比较,血气相关指标二氧化碳分压(PaCO_(2))、氧分压(PaO_(2))、肺动脉血氧饱和度(SaO_(2))逐渐下降;血常规指标白细胞(WBC)、红细胞计数(RBC)和血小板(PLT)均显著增加;血生化指标血糖水平显著升高,白蛋白(ALB)、血清尿素氮(BUN)异常升高,肌酸激酶(CK)、肌酸激酶同工酶(CK-MB)显著降低(P<0.01或P<0.05)。结论模拟高原低压性缺氧不同时间大鼠的血液学指标均发生显著改变,严重影响大鼠的正常生理活动。

Objective To investigate the effects of simulated plateau acute decompression hypoxia on blood indexes in rats.Method Forty healthy male Wistar rats were randomly divided into normoxia control group,hypoxia 10,20,30 h group,10 rats in each group.were put into hypobaric hypoxia treatment to simulate the low-pressure and hypoxic environment at 8000 m altitude.Normal rats were kept at 1500 m above sea level,the other 3 groups stayed at simulated altitude of 8000 m for 10,20 and 30 h respectively by large low pressure and low oxygen animal laboratory.Check blood sugar,blood gas,blood routine,and blood biochemistry.Observe the effects of different hypoxia time on hematological indexes of rats.Result With the prolongation of hypoxia time,the blood glucose of the rats also increased;According to the blood gas index,the partial pressure of carbon dioxide(PaCO_(2)),partial pressure of oxygen(PaO_(2)),pulmonary oxygen saturation(SaO_(2))gradually decrease with the prolongation of hypoxia;Blood routine result showed that white blood cells(WBC),red blood cell count(RBC)and platelet(PLT)in rat arterial blood increased significantly with the prolongation of hypoxia;blood biochemical result showed that albumin(ALB),serum urea nitrogen(BUN)were abnormally elevated.creatine kinase(CK),and creatine kinase isoenzyme(CK-MB)were significantly decreased(P<0.01 or P<0.05).Conclusion The hematological parameters of rats in different periods of simulated plateau hypoxia were changed,which seriously affected the normal physiological activities of rats.