新型镁合金黏膜刺激性、急性全身毒性及抗菌性能研究

Study on mucosal irritation,acute systemic toxicity and antibacterial properties of the novel magnesium alloys

目的测定新型镁合金(Mg-3Sn-0.5Mn)的黏膜刺激性、急性全身毒性及抗菌性能。方法通过金黄地鼠黏膜刺激实验及小鼠急性全身毒性实验,测试新型镁合金的生物相容性;通过镁合金与菌液(大肠杆菌和金黄色葡萄球菌)共培养1 d和3 d后平板计数及扫描电镜观察合金表面细菌状态,测试新型镁合金的抗菌性能。结果小鼠急性全身毒性实验:72 h观察期内实验组和对照组小鼠均未出现任何急性毒性反应;黏膜刺激实验:14 d后实验组与对照组金黄地鼠颊黏膜大体观察及HE染色无明显异常;抗菌实验:实验组镁合金与对照组316不锈钢相比,培养皿表面菌落数量明显较少,抗菌效果明显;扫描电镜观察:镁合金表面金黄色葡萄球菌数量明显少于对照组,且形态呈明显皱缩状。结论新型镁合金(Mg-3Sn-0.5Mn)具有良好的生物相容性及抗菌性能,可初步满足作为医用材料的基本要求。

Objective To determinethe mucosal irritant,acute systemic toxicity and antibacterial propertiesof novel magnesium alloys(Mg-3Sn-0.5Mn).Methods Through the mucosal stimulation experiment of golden gopher and the acute toxicity experiment of mice,the biocompatibility of the new magnesium alloy was tested.The bacterial state on the surface of the new magnesium alloy was observed by plate counting and scanning electron microscopy after the culture of magnesium alloy and bacterial liquid(Escherichia coli and Staphylococcus aureus)for 1 d and 3 d.Results Acute systemic toxicity experiment of mice showed that during the 72 h observation period,there was no acute toxicity reaction in the experimental group and the mice in the control group.Mucosal stimulation experiment showed that after 14 d,there was no obvious abnormality between the experimental group and the control group in the cheek mucosa HE staining and gross observation of the golden gopher.Antibacterial experiment revealed that compared with 316 stainless steel in the control group,the number of bacterial colonies on the surface of the petri dish was significantly smaller,and the antibacterial effect was obvious.According to scanning electron microscope observation,the number of Staphylococcus aureus on the surface of magnesium alloy was significantly less than that of the control group,and the shape was obviously wrinkled.Conclusion The new magnesium alloy(Mg-3Sn-0.5Mn)has good biocompatibility and antibacterial properties,which can initially meet the basic requirements of medical materials.