Th1/Th2免疫平衡偏移介导JAK2-STAT3信号通路参与感染性早产发生机制研究

Study on the Mechanism of JAK2-STAT3 Signaling Pathway Mediated by Th1/Th2 Immune Balance Shift in the Occurrence of Infectious Preterm Birth

目的:探讨Th1/Th2免疫平衡偏移情况及JAK2-STAT3信号通路参与感染性早产发生机制。方法:选取就诊于大理大学第一附属医院的60例早产患者为研究对象(早产组),根据产后胎膜组织病理检查结果分为感染性早产组和非感染性早产组,选取同期正常足月分娩的产妇20例为正常对照(正常对照组)。采用酶联免疫吸附测定试剂盒检测各产妇外周血清中干扰素-γ(IFN-γ)、白细胞介素-4(IL-4)、白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)的表达水平,并计算Th1/Th2免疫调节平衡指数;采用免疫组织化学染色法检测胎膜组织中信号转导及转录激活因子3(STAT3)、核因子κB(NF-κB)蛋白的表达情况。结果:IFN-γ、TNF-α表达水平在早产组中显著升高(P<0.05),尤其是在感染性早产组中升高更明显,Th1/Th2免疫平衡具有明显向Th1方向偏移趋势;IL-6表达水平、STAT3及NF-κB蛋白表达水平在感染性早产组中显著升高(P<0.05),非感染性早产组与正常对照组比较差异无统计学意义(P>0.05)。结论:Th1/Th2免疫平衡可通过TNF-α、NF-κB、IL-6、JAK2-STAT3信号通路参与感染性早产的发生。

Objective:To investigate the Th1/Th2 immune imbalance and the involvement of the JAK2-STAT3 signaling pathway in the mechanism of infectious preterm birth.Methods:A total of 60 preterm patients treated at the First Affiliated Hospital of Dali University were selected as the research objects(preterm group).Based on the results of postpartum fetal membrane tissue pathological examination,the preterm group was divided into the infectious preterm birth group and non-infectious preterm birth group.20 puerperae who had full-term deliveries during the same period were selected as the control group.The expression levels of interferon-γ(IFN-γ),interleukin-4(IL-4),interleukin-6(IL-6)and tumor necrosis factor-α(TNF-α)in the peripheral blood of the puerperae in the three groups were detected by ELISA kits,and the Th1/Th2 immune regulatory balance index was calculated.The expressions of STAT3 and nuclear factor-κB(NF-κB)proteins in fetal membrane tissue were detected by immunohistochemistry.Results:The expression levels of IFN-γand TNF-αwere significantly elevated in the preterm group(P<0.05),especially in the infectious preterm birth group,indicating that the Th1/Th2 immune balance had a significant shift toward Th1.The expression levels of IL-6,STAT3,and NF-κB proteins were significantly higher in the infectious preterm birth group(P<0.05),while there was no significant difference between the non-infectious preterm birth group and the control group(P>0.05).Conclusion:The Th1/Th2 immune balance may be involved in the occurrence of infectious preterm birth through TNF-α,NF-κB,IL-6 and JAK2-STAT3 signaling pathways.