CyclinD1、p27在非小细胞肺癌中的表达及与其临床病理特征及预后的关系

Expression of CyclinD1 and p27 in NSCLC and Its Relationship with Clinicopathological Features and Postoperative Recurrence

目的探讨细胞周期蛋白D1(CyclinD1)、p27在非小细胞肺癌(NSCLC)中的表达及与其临床病理特征、术后复发的关系。方法选取92例NSCLC患者,采用免疫组织化学SP法检测癌组织和癌旁组织中的CyclinD1、p27水平。结果NSCLC癌组织中CyclinD1阳性率[72.83.%(67/92)]较癌旁组织[23.91%(22/92)]高,p27阳性率[16.30%(15/92)]较癌旁组织[60.87%(56/92)]低(P<0.05);低分化、Ⅲ期、有LNM的NSCLC患者CyclinD1阳性率[96.00%(24/25)、93.88%(46/49)、97.44%(38/39)]较中高分化[64.18%(43/67)]、Ⅰ~Ⅱ期[48.84%(21/43)]、无LNM[54.72%(29/53)]高(P<0.05);中高分化、Ⅰ~Ⅱ期、无LNM的NSCLC患者p27阳性率[22.39%(15/67)、30.23%(13/43)、24.53%(13/53)]较低分化[0.00%(0/25)]、Ⅲ期[4.08%(2/49)]、有LNM[5.13%(2/39)]高(P<0.05);复发NSCLC患者癌组织中CyclinD1阳性率[89.47%(51/57)]较未复发患者[45.71%(16/35)]高,p27阳性率[5.26%(3/57)]较未复发患者[34.29%(12/35)]低(P<0.05)。结论CyclinD1在NSCLC中的阳性率高,p27在NSCLC中的阳性率低,且二者与NSCLC分化程度、疾病分期等临床病理特征和术后复发存在明显关联性,临床可将CyclinD1、p27指标作为评估NSCLC进展和预后的重要指标进行检测,为临床治疗提供参考。

Objective To investigate the expression of cyclinD1 and p27 in non-small cell lung cancer(NSCLC)and their relationship with clinical pathological features and postoperative recurrence.Methods 92 NSCLC patients were selected and the levels of CyclinD1 and p27 in cancer tissue and adjacent tissues were detected using immunohistochemical SP method.Results The positive rate of CyclinD1 in NSCLC cancer tissue[72.83%(67/92)]was higher than that in adjacent tissue[23.91%(22/92)],while the positive rate of p27[16.30%(15/92)]was lower than that in adjacent tissue[60.87%(56/92)](P<0.05);The positive rates of CyclinD1 in NSCLC patients with poorly differentiated stageⅢand LNM[96.00%(24/25),93.88%(46/49),97.44%(38/39)]were higher than those in moderately well differentiated stageⅢ,stageⅠ-Ⅱ[48.84%(21/43)],and non LNM[54.72%(29/53)](P<0.05);The positive rates of p27 in NSCLC patients with medium to high differentiation,stageⅠ-Ⅱ,and no LNM were[22.39%(15/67),30.23%(13/43),and 24.53%(13/53)],which were higher than those with low differentiation[0.00%(0/25)],stageⅢ[4.08%(2/49)],and LNM[5.13%(2/39)](P<0.05);The positive rate of CyclinD1 in cancer tissue of recurrent NSCLC patients[89.47%(51/57)]was higher than that of non recurrent patients[45.71%(16/35)],and the positive rate of p27[5.26%(3/57)]was lower than that of non recurrent patients[34.29%(12/35)](P<0.05).Conclusion The positive rate of CyclinD1 is high in NSCLC,while the positive rate of p27 is low in NSCLC.There is a significant correlation between the two and clinical pathological features such as differentiation degree,disease stage,and postoperative recurrence of NSCLC.CyclinD1 and p27 indicators can be used as important indicators for evaluating the progression and prognosis of NSCLC in clinical practice,providing reference for clinical treatment.