刘寄奴水煎剂联合虾青素对动脉粥样硬化大鼠抗氧化、抗炎作用的研究

Study on the antioxidant and anti-inflammatory effects of the Liujinu decoction combined with astaxanthin on AS rats

目的:探讨刘寄奴水煎剂联合虾青素对动脉粥样硬化(Atherosclerosis,AS)大鼠血脂、抗氧化水平和炎症因子的影响。方法:将60只健康雄性SD大鼠随机分为空白组、模型组、阿托伐他汀组、中药低剂量组、中药中剂量组、中药高剂量组,每组10只。采用高脂饲料+腹腔注射维生素D3建立AS模型。造模成功后,各组灌胃给药干预4周。ELISA检测各组大鼠血清血脂水平[三酰甘油(Triacylglycerol,TG)、总胆固醇(Total Cholesterol,TC)、低密度脂蛋白胆固醇(Low-density Lipoprotein Cholesterol,LDL-C)]、抗氧化水平[超氧阴离子(O2-)、羟自由基(-OH)、一氧化氮(Nitric Oxide,NO)、超氧化物歧化酶(Superoxide Dismutase,SOD)、丙二醛(Malondialdehyde,MDA)、谷胱甘肽过氧化物酶(Glutathione Peroxidase,GSH-Px)]、炎症因子水平[白细胞介素(Interleukin,IL)-6、超敏C反应蛋白(Hypersensitive C-reactive Protein,hs-CRP)、肿瘤坏死因子-α(Tumor Necrosis Factor-α,TNF-α)]。结果:与模型组相比,中药低剂量组、中药中剂量组、中药高剂量组大鼠血清TC、TG、LDL-C水平降低、HDL-C水平升高,且呈剂量依赖性,其中中药中剂量组、中药高剂量组差异有统计学意义(P<0.01或P<0.05);血清O2-、-OH、MDA水平均降低,SOD、GSH-Px和NO水平均升高,且呈剂量依赖性,中药中剂量组、中药高剂量组差异均有统计学意义(P<0.01或P<0.05);血清IL-6、IL-1β、hs-CRP、TNF-α水平均降低,差异有统计学意义(P<0.01或P<0.05)。结论:刘寄奴水煎剂联合虾青素可降低AS大鼠血脂水平、炎症反应水平,增强抗氧化水平,且呈剂量依赖性。

Objective:To investigate the effects of the Liujinu decoction(刘寄奴水煎剂)combined with astaxanthin on blood lipids,antioxidant levels and inflammatory factors in rats with Atherosclerosis(As).Methods:Sixty healthy male SD rats were randomly divided into the blank group,model group,atorvastatin group,the Liujinu decoction combined with astaxanthin low-dose,medium-dose and high-dose groups,with 10 rats in each group.The AS model was established by high fat diet and intraperitoneal injection of VD3.After successful modeling,each group was given drugs by gavage for 4 weeks.Serum lipid levels(TC,TG,LDL-C,HDL-C),antioxidant levels(O2-,-OH,NO,SOD,MDA,GSH-Px)and inflammatory factors(IL-6,IL-1β,hs-CRP,TNF-a)were detected by ELISA.Results:Compared with the model group,the serum levels of TC,TG and LDL-C were decreased and the levels of HDL-C were increased in the low,medium and high dose groups,and the differences were statistically significant(P<0.01 or P<0.05).The levels of serum O2-,-OH and MDA were decreased,while the levels of SOD,GSH-Px and NO were increased in a dose-dependent manner,and the differences between the middle and high dose groups were statistically significant(P<0.01 or P<0.05).The serum levels of IL-6,IL-1β,hs-CRP and TNF-αwere decreased,and the differences were statistically significant(P<0.01 or P<0.05).Conclusion:The Liujinu decoction combined with astaxanthin can reduce the level of blood lipid and inflammatory response,and enhance the level of antioxidant in a dose-dependent manner in atherosclerotic rats.