用于检测多硫化氢的荧光探针研究进展

Advances in fluorescence probes for detection of hydrogen polysulfides

多硫化氢(hydrogen polysulfides,H2Sn,n≥2)作为硫化氢(hydrogen sulfide,H2S)所衍生的活性硫物质,在生理调节及信号转导过程中扮演着不可或缺的角色。H2Sn与H2S互为氧化还原对,一般经氧化或酶促作用从H2S转化生成,通过调节蛋白质相互作用及改变酶活力等方式发挥其生理作用。随着研究的深入,发现一些原本被认为是H2S发挥的生理学功能可能是由H2Sn所发挥的,并且H2Sn具有更高的蛋白质巯基化效率。因此,实时检测生物体内的H2Sn对于研究其生理活性及与H2S之间的联系具有十分重要的意义。传统的质谱光谱等检测方法需要破碎组织细胞,不适用于生物体内的实时检测。而荧光探针因其具有高灵敏度及特异性,同时生物毒性低常被选作H2Sn原位实时检测工具。本文概述了H2Sn的生理调节活性,着重基于响应机制的反应类型的介绍H2Sn检测荧光探针的设计策略、荧光性能、检测特点及生物成像应用等方面,并对这一领域所面临的挑战与发展进行了展望。

Sulfane sulfur species in the reactive sulfur species family include hydrogen polysulfides(H2Sn,n≥2),which play an essential role in physiological regulation and signal transduction.As a redox pair of H2S,H2Sn can be produced through oxidation or enzyme reaction and regulate protein interaction and enzyme activity.Research has revealed that H2Sn,with higher efficiency of protein S-sulfhydration than H2S,may be responsible for some physiological functions previously attributed to H2S.Therefore,real-time detection of H2Sn is crucial for studying its physiological activity and the relationship between H2S and H2Sn.Traditional detection methods,such as mass spectrometry,are not suitable for living organisms as they require tissue cell disruption.Instead,fluorescence probes are often used for in situ real-time detection due to their high sensitivity and specificity and low biological toxicity.This review summarizes the physiological regulatory activity of H2Sn,as well as the design strategy,response mechanism,fluorescence characteristics,and biological applications of H2Sn fluorescent probes based on the structure of the response group,with a prospect of the challenges and developments in this field.