基于网络药理学探讨柴胡-白术药对治疗阿尔茨海默病的作用机制

The Mechanism of Bupleurum-Atractylodes in the Treatment of Alzheimer's Disease Based on Network Pharmacology

目的:基于网络药理学方法探讨柴胡-白术药对治疗阿尔茨海默病的作用机制。方法:通过TCMSP平台筛选出柴胡、白术的活性成分及靶点,挖掘GeneCards等数据库与阿尔茨海默病的相关靶点,并利用Cytoscape 3.7.1软件构建药物-有效成分-靶点网络图,利用CytoNCN进行网络拓扑分析,找到柴胡-白术药对治疗阿尔茨海默病的关键靶点,进行蛋白-蛋白互作(PPI)网络及活性成分-阿尔茨海默病作用靶点网络的构建。采用基因本体(GO)功能富集分析和京都基因与基因组百科全书(KEGG)通路富集分析柴胡-白术药对治疗阿尔茨海默病的潜在作用机制。结果:共获得16种主要活性成分、167个化合物靶点、8730个与阿尔茨海默病发病相关的靶基因。利用网络拓扑分析最终筛选出40个关键靶点,GO富集分析得到313条信号条目,KEGG富集分析得到104条信号通路。结论:初步验证和预测柴胡-白术药对治疗阿尔茨海默病多成分、多靶点、多途径的作用特点,为进一步探讨其作用机制提供思路与参考。

Objective:To explore the mechanism of Bupleurum-Atractylodes in the treatment of Alzheimer's disease based on network pharmacology.Methods:The active components and targets of Bupleurum and Atractylodes were screened out by TCMSP platform,the related targets of GeneCards database and Alzheimer's disease were mined,and drug-active ingredient-target network diagram were constructed using the Cytoscape 3.7.1 software.The key targets of Bupleurum-Atractylodes in the treatment of Alzheimer's disease were found by the network topology analysis using CytoNCN.The construction of protein-protein interactions(PPI)network and active ingredient-Alzheimer's disease action target network were constructed.Gene Ontology(GO)functional enrichment analysis and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment were used to analyze the potential mechanism of Bupleurum-Atractylodes in the treatment of Alzheimer's disease.Results:A total of 16 main active ingredients,167 compound targets,and 8730 target genes associated with the development of Alzheimer's disease were obtained.Forty key targets were finally screened using network topology analysis,and 313 signaling entries were obtained from GO function enrichment analysis and 104 signaling pathways from KEGG pathway enrichment analysis.Conclusion:The preliminary validation and prediction of the action characteristics of Bupleurum-Atractylodes in the treatment of Alzheimer's disease with multi-components,multi-targets,and multi-pathways were achieved,which provided ideas and references for further exploring its mechanism.