蔗糖和咖啡因的持续摄入对不同饮食小鼠生理代谢的影响

Effects of the Sustainable Intake of Sucrose and Caffeine on the Physiological Metabolism of Mice Fed with Different Diets

通过对市面上的奶茶产品进行调研和测定,结合人们日常消费习惯,以奶茶中总糖和咖啡因浓度的中位数为标准灌胃小鼠。在常规饲料和高糖高脂饲料喂养的基础上,使用C57BL/6J雄性小鼠模拟人每天喝1杯奶茶摄入的蔗糖和咖啡因量,以体重、血糖、血脂、炎症因子和组织学检查作为评价指标,研究蔗糖和咖啡因的持续摄入对小鼠生理代谢的影响。实验结果表明:所选取奶茶样品中总糖质量浓度范围为25~135 g/L,咖啡因质量浓度范围为190~853 mg/L;与常规饲料组相比,常规饲料加蔗糖和咖啡因组空腹血糖值由(4.27±0.06)mmol/L上升至(5.43±0.39) mmol/L,血清肿瘤坏死因子-α质量浓度由(89.48±2.85) ng/L上升至(112.94±1.76)ng/L,血清白细胞介素-6质量浓度由(49.31±3.23)ng/L上升至(65.44±4.70)ng/L,体重增加值由(3.33±0.64)g下降至(2.45±0.51)g,血清甘油三酯浓度由(0.88±0.29)mmol/L下降至(0.74±0.04)mmol/L;与高糖高脂饲料组相比,高糖高脂饲料加蔗糖和咖啡因组体重增加值由(8.38±1.42)g下降至(3.67±1.23)g,血清甘油三酯浓度由(0.86±0.07)mmol/L下降至(0.71±0.06)mmol/L。长期摄入蔗糖和咖啡因混合溶液能使小鼠体重和血清甘油三酯下降,但会使空腹血糖和促炎因子增加。

By referring to the concentration of sugar and caffeine in bubble tea from popular brands on the market,combined with people's daily consumption habits,we used the median concentration of sugar and caffeine in bubble tea as the standard to gavage mice.The C57 BL/6J male mice were used to be fed with a normal diet or a high-sugar and high-fat diet to mimic humans’dietary habit of drinking a cup of bubble tea every day.The body weight,blood glucose,blood lipids,inflammatory factors,and histological changes were measured after the treatments to study the effect of the sustainable intake of sucrose and caffeine on the physiological metabolism of mice.The results showed that in the bubble tea selected,the mass concentration of sugar ranged from 25 g/L to 135 g/L,and that of caffeine ranged from 190 mg/L to 853 mg/L.Comparing to the normal diet group,that supplemented with additional sucrose and caffeine resulted in an increased concentration of fasting blood glucose(FBG)from(4.27±0.06)mmol/L to(5.43±0.39)mmol/L,an increased concentration of serum tumor necrosis factor-αfrom(89.48±2.85)ng/L to(112.94±1.76)ng/L,an increased concentration of serum interleukin-6 from(49.31±3.23)ng/L to(65.44±4.70)ng/L,a decreased weight gain from(3.33±0.64)g to(2.45±0.51)g,and a decreased concentration of serum triglyceride from(0.88±0.29)mmol/L to(0.74±0.04)mmol/L.Comparing to the high-sugar and high-fat diet group,that supplemented with additional sucrose and caffeine resulted in a decreased weight gain from(8.38±1.42)g to(3.67±1.23)g,and a decreased concentration of serum triglyceride from(0.86±0.07)mmol/L to(0.71±0.06)mmol/L.Long-term intake of sucrose and caffeine can lead to a decrease in body weight and serum triglyceride,but an increase in the concentration of FBG and pro-inflammatory factors.