大黄蒽醌对大鼠糖皮质激素性股骨头坏死的保护作用

Protective effect of rhubarb anthraquinone on steroid induced avascular necrosis of femoral head

目的观察大黄蒽醌对大鼠糖皮质激素性股骨头坏死的保护作用。方法162只SD雌性大鼠随机分为正常组、模型组、实验组,分别给予生理盐水、醋酸泼尼松(3.5 mg/kg)、大黄蒽醌(70 mg/kg)+醋酸泼尼松(3.5 mg/kg)灌胃13周。每组在3、5、7、9、11、13周处死9只大鼠,进行股骨头Micro CT扫描及重建、骨形态计量学分析。结果MicroCT重建显示在3周时3组骨小梁结构近乎相似;13周时,模型组骨小梁结构遭到严重破坏,全骨结构特性破坏,实验组接近于正常组。与正常组比较,模型组与实验组在3、5、7、9、11周骨小梁骨密度(Tb.BMD)、骨体积分数(BV/TV)、骨小梁厚度(Tb.Th)、骨小梁数量(Tb.N)水平下降,结构模型指数(SMI)、骨小梁分离度(Tb.Sp)升高,且模型组更为显著(P<0.01或0.05);13周时,实验组Tb.BMD、BV/TV、Tb.Th、Tb.N水平高于模型组,SMI、Tb.Sp水平低于模型组(P<0.01)。结论大黄蒽醌对大鼠糖皮质激素性股骨头坏死有预防作用。

Objective To investigate the protective effect of rhubarb anthraquinone(RA)on steroid induced avascular necrosis of femoral head(SANFH).Methods A total of 162 female SD rats were randomly divided into normal,model and experimental groups,each receiving normal saline,prednisone acetate(3.5 mg/kg)or RA(70 mg/kg)+prednisone acetate(3.5 mg/kg)for 13 weeks.At 3,5,7,9,11 and 13 weeks,9 rats in each group were sacrificed for Micro CT scanning and reconstruction of the femoral head and morphometry analysis of the femur.Results Micro-CT reconstruction showed that the trabecular structure was nearly similar among 3 groups at 3 weeks.At 13 weeks,the trabecular structure was severely damaged with whole bone destruction in model group,and comparable between experimental and normal groups.At 3,5,7,9,and 11 weeks,trabecular bone mineral density(Tb.BMD),bone volume/total volume(BV/TV),trabecular thickness(Tb.Th),and trabecular number(Tb.N)were decreased,while structural model index(SMI)and trabecular separation(Tb.Sp)increased(P<0.01 or 0.05)in model and experimental groups compared with normal group,especially in model group.At 13 weeks,Tb.BMD,BV/TV,Tb.Th and Tb.N were higher,while SMI and Tb.Sp were lower in experimental group compared with model group(P<0.01).Conclusion RA can prevent SANFH in rats.