16S rDNA测序分析妊娠期细菌性阴道病患者的阴道菌群结构组成

16S rDNA Sequencing Analyzed the Structural Composition of the Vaginal Flora of Patients with Bacterial Vaginosis During Pregnancy

目的本研究主要通过对健康孕妇与妊娠期细菌性阴道病(bacterial vaginosis,BV)患者的阴道分泌物进行检测,试图阐述不同孕期BV患者的阴道菌群组成。方法选取在濮阳市妇幼保健院产科门诊诊断为妊娠期BV的孕早期患者15例、孕晚期患者24例及孕早期、孕晚期健康孕妇各30例。所有样本选择16S rDNA V3V4可变区进行测序分析,比较各组之间阴道微生物构成的差异。结果孕早期健康孕妇组与孕早期BV组之间的Alpha多样性比较,差异有统计学意义(P<0.05),孕晚期健康孕妇组与孕晚期BV组之间的Alpha多样性比较,无显著统计学差异。共发现26个门,各组均以厚壁菌门、放线菌门、拟杆菌门占主要比例。在属水平上,共发现51个属,孕早期健康孕妇组相对丰度最高的是乳杆菌属(约占87%)。孕晚期健康孕妇组相对丰度最高的是乳杆菌(约占97%)。BV患者乳杆菌明显减少,阿托波菌属,巨球型菌属,普氏菌属,需氧球菌属,纤毛菌属明显增多。结论健康孕妇阴道菌群以乳杆菌占绝对优势,大部分以瑞士乳杆菌或惰性乳杆菌为主,还存在普氏菌属,双歧杆菌属,链球菌属等差异菌属;大部分BV患者阴道菌群表现为乳杆菌显著减少甚至缺失。

Objective This study mainly examines the vaginal secretions of healthy pregnant women and patients with bacterial vaginosis(bacterial vaginosis,BV)in pregnancy,and tries to explain the vaginal flora composition of different pregnant BV patients.Methods 15 patients in early pregnancy,24 patients in late pregnancy and 30 healthy pregnant women in early pregnancy were selected in the obstetric clinic of Puyang Maternal and Child Health Hospital.For all samples,the 16S rDNA V3V4 variable region was selected for sequencing analysis to compare the differences in vaginal microbial composition between the groups.Results The Alpha diversity comparison between healthy pregnant women and BV group was statistically significant(P<0.05),and there was no significant statistical difference in Alpha diversity comparison between healthy pregnant women and BV group.A total of 26 phyla were found,including Firmicutes,Actinobacteria and Bacteroidetes.At the genus level,51 genera were found,and the highest relative abundance of the early pregnancy healthy pregnant women group was Lactobacillus(about 87%).The highest relative abundance in late trimester healthy pregnant women was Lactobacillus(about 97%).BV patients significantly decreased lactobacillus,atorbol genus,macrococcus,Prevotella,oxycoccus,and ciliella.Conclusion The vaginal flora of healthy pregnant women is absolutely dominant,most of them are L.suiss or Lactobacillus inert,including Prevotella,Bifidobacterium and Streptococcus;most BV patients'vaginal flora is significantly reduced or even absent.