摘要
目的:采用高效液相色谱法测定达原饮中槟榔生物碱的含量。方法:色谱条件为:Venusil SCX色谱柱(4.6 mm×250 mm,5μm,300Å),流动相:甲醇:0.2%磷酸(氨水调节pH值至3.8)=60:40,检测波长:210 nm,柱温:30℃,流速:1.0 mL•min^(-1)。结果:4种槟榔生物碱分离度良好,各组分在浓度范围内线性关系良好,加标回收率(n=9)在98.37%~102.11%,RSD值均小于2%。达原饮药液中去甲槟榔碱、槟榔碱、去甲槟榔次碱和槟榔次碱的含量分别为0.014 mg•mL^(-1)、0.046 mg•mL^(-1)、0.036 mg•mL^(-1)和0.018 mg•mL^(-1)。结论:该方法准确度高、稳定性强、灵敏度和重复性良好,简便可靠,可用于达原饮中槟榔生物碱的含量测定及质量控制。
Objective:A method for the determination of areca alkaloids in Dayuanyin was established by HPLC.Methods The determination was performed on A Venusil SCX column(4.6 mm×250 mm,5µm,300 A)with mobile phase consisting of methanol:0.2%phosphoric acid(pH adjusted to 3.8 by ammonia)=60:40.Detection wavelength was set at 210 nm,column temperature at 30℃,and flow rate at 1.0 mL·min^(-1).Results The four areca alkaloids were well separated,and the linear relationship of each component was good in the concentration range.The standard recoveries(n=9)ranged from 98.37%to 102.11%,and RSD were all less than 2%.The contents of guvacoline,arecoline,guvacine hydrochloride and arecaine in Dayuan decoction were 0.014 mg·mL^(-1),0.046 mg·mL^(-1),0.036 mg•mL^(-1)and 0.018 mg·mL^(-1),respectively.Conclusion The method has high accuracy,stability,sensitivity and repeatability,is simple and reliable,and can be used for the content determination and quality control of areca alkaloids in Dayuan drink.
作者
张莹
刘冰
陈秀梅
黄涛
杨芳雨
张博雄
Zhang Ying;Liu Bing;Chen Xiu-mei;Huang Tao;Yang Fang-yu;Zhang Bo-xiong(School of Medicine,Xiangyang Polytechnic,Xiangyang Hubei 441050,China)
出处
《江西化工》
CAS
2023年第5期29-32,共4页
Jiangxi Chemical Industry
基金
湖北省教育厅科学研究计划项目(No.B2021486)
襄阳市医疗卫生领域科技计划项目重点项目(2021YL50)。
关键词
达原饮
槟榔
生物碱
高效液相色谱法
Dayuan Yin
areca nut
alkaloid
high performance liquid chromatography