高效液相色谱法应用于消炎镇痛膏中颠茄流浸膏的质量控制

Quality Control of Belladonna Liquid Extract in Xiaoyan Zhentong Ointment by HPLC

目的:建立高效液相色谱法用于消炎镇痛膏中颠茄流浸膏的质量控制,为科学评价及有效控制消炎镇痛膏中颠茄流浸膏真实投料提供可靠方法。方法:以十八烷基硅烷键合硅胶为填充剂(Intersil ODS-SP C18色谱柱,柱长为25cm,内径为4.6mm,粒径为5μm);以乙腈为流动相A,以0.4%磷酸溶液为流动相B,进行梯度洗脱;检测波长为344nm;柱温为25℃;流速为每分钟1.0ml。结果:采用高效液相色谱法,能够容易、快速地鉴别消炎镇痛膏中颠茄流浸膏特征性成分。结论:该方法简便、准确、阴性无干扰,可有效地提高消炎镇痛膏质控水平,控制方中颠茄流浸膏真实投料。

Objective:To establish an HPLC method for the quality control of Belladonna Liquid Extract in Xiaoyan Zhentong Ointment.To provide a reliable method for scientific evaluation and effective control of the true feeding of Belladonna Liquid Extract in Xiaoyan Zhentong Ointment.Methods:Octadecylsilane bonded silica gel(Intersil ODS-SP C18 column,25 cm in length,4.6 mm in diameter,5μm in particle size)was used as the packing agent;acetonitrile was used as the mobile phase A,and 0.4%phosphoric acid solution was used as the mobile phase B for gradient elution;the detection wavelength was 344nm;the column temperature was 25℃;the flow rate was 1.0 ml/min.Results:the characteristic components of Belladonna Liquid Extract in Xiaoyan Zhentong Ointment could be identified easily and quickly by HPLC.Conclusion:the method is simple,accurate,negative and non-interference,which can effectively improve the quality control level of Xiaoyan Zhentong Ointment and control the real dosage of semen Ziziphi Spinosae.