小麦品种红农1号抗条锈性遗传分析

Genetic analysis of stripe rust resistance in Hongnong No.1 wheat

小麦条锈病是危害小麦产量和品质最严重的病害之一,选育新抗条锈病品种进行抗条锈基因合理布局可控制小麦条锈病,本文利用抗病性鉴定方法评价苗期和成株期红农1号对流行小麦条锈菌生理小种的抗病性,运用SPSS 20.0统计软件进行χ2检验检测其适合度,确定抗病基因数目、显隐性及遗传方式,并对CYR34的抗条锈菌基因进行遗传分析。结果表明:苗期红农1号对条锈菌生理小种CYR31、CYR32、CYR34均表现为抗病,尤其是对CYR31和CYR34表现为近免疫;田间接种CYR34成株期红农1号表现为近免疫。红农1号/高原448杂交群体中苗期F1代均表现高抗,F2代中240株表现抗病,75株表现感病,符合3∶1的抗/感分离比例;成株期F1代表现高抗,F2代中218株表现抗病,58株表现感病,符合3∶1的抗/感分离比例。综上可知,红农1号对CYR34具有全生育期抗性,其抗性由1对显性基因控制。该研究结果为红农1号抗病基因的定位和抗病育种利用奠定了基础。

Wheat stripe rust is one of the most serious diseases affecting the yield and quality of wheat.Therefore,in order to select new varieties with resistance to stripe rust and arrange resistance genes reasonably so as to control wheat stripe rust,disease resistance identification method is used to evaluate the resistance of Hongnong No.1 to the physiological races of popular wheat stripe rust during the seedling and adult stages.Statistical software SPSS 20.0 is utilized for testing the suitability ofχ~2.The number of disease resistance genes,dominant and recessive genes as well as genetic mode is determined.In addition,the genetic analysis of CYR34 resistance genes to stripe rust is conducted.The results show that Hongnong No.1 exhibits resistance to the physiological races CYR31,CYR32 and CYR34 of stripe rust during the seedling stage,especially near immunity to CYR31 and CYR34;the field inoculation of CYR34 in the adult stage of Hongnong No.1 shows near immunity.The F_1 gene-ration of the hybrid population of Hongnong No.1/Gaoyuan 448 shows high disease resistance during the seedling stage,with 240 plants resistant and 75 plants susceptible in the F_2 gene-ration,which is consistent with a 3∶1 separation ratio of resistance/susceptibility.During the adult stage,F_1 generation shows high resistance,218 plants of F_2 generation shows resistance,and 58 shows susceptibility,which is consistent with a 3∶1 separation ratio of resistance/susceptibility.In summary,it can be seen that Hongnong No.1 has full growth period resistance to CYR34,and its resistance is controlled by a pair of dominant genes.The results lays a foundation for the localization of disease resistance genes in Hongnong No.1 and its utilization in disease resistance breeding.