苏中苏南地区冠心病患者CYP2C19基因多态性分析

CYP2C19 polymorphisms in patients with coronary heart disease in the central and southern Jiangsu

目的 探讨冠心病患者CYP2C19基因在苏中苏南地区分布的多态性,比较其在不同地域人群中的分布,为冠心病患者合理使用药物提供指导。方法 选择2019年8月至2022年12月扬州大学临床医学院和苏州大学附属第一医院收治且完善CYP2C19基因多态性检测的2 926例冠心病患者为研究对象。利用焦磷酸测序仪对患者CYP2C19相关基因位点进行测序,并比较CYP2C19基因多态性在不同地区的分布差异。采用SPSS 26.0统计软件进行数据分析。根据数据类型,分别采用t检验或χ2检验进行组间比较。结果 2 926例患者根据基因型分为快代谢型(*1/*1),中代谢型(*1/*2、*1/*3)和慢代谢型(*2/*2、*2/*3和*3/*3),频率分别为38.21%(1 118/2 926)、45.80%(1 340/2 926)和15.99%(468/2 926)。冠心病患者的CYP2C19等位基因和基因型分布在不同性别之间差异无统计学意义(P>0.05)。苏中苏南地区冠心病患者CYP2C19等位基因分布与陕西比较,差异有统计学意义(P<0.05)。苏中苏南地区冠心病患者CYP2C19代谢型分布与陕西、广州、淮海、河南比较,差异有统计学意义(P<0.05)。结论 苏中苏南地区冠心病患者CYP2C19基因多态性的分布存在差异,主要为中快代谢型,且不同性别间无明显差异,此可为冠心病患者精准用药提供指导。

Objective To investigate the CYP2C19 polymorphism in the patients with coronary heart disease(CHD)in the central and southern Jiangsu province of China and to compare its distribution in different geographical populations,so as to provide guidance for the rational use of drugs in CHD patients.Methods A total of 2926 patients with coronary heart disease who were admitted to Clinical Medical College of Yangzhou University and First Affiliated Hospital of Soochow University from August 2019 to December 2022,and have completed CYP2C19 gene polymorphism detection,were selected as the study objects.The CYP2C19 gene loci were sequenced by pyrosequencer,and its polymorphism distribution in different regions was compared.SPSS statistics 26.0 was used for data analysis.According to the data type,t test orχ2 test was used for comparison between groups.Results A total of 2926 patients were enrolled in this study.According to the genotype,they were divided into three groups:extensive metabolizer(*1/*1),intermediate metabolizer(*1/*2,*1/*3),and poor metabolizer(*2/*2,*2/*3 and*3/*3),with a frequency of 38.21%(1118/2926),45.80%(1340/2926),and 15.99%(468/2926),respectively.There was no statistically significant difference in the distribution of CYP2C19 alleles and genotypes between male and female CHD patients(P>0.05).The distribution of CYP2C19 allele in CHD patients in central and southern Jiangsu Province was significantly different from that in Shaanxi Province(P<0.05).There was statistical significance in the distribution of CYP2C19 metabolic types in CHD patients in central and southern Jiangsu Province compared with Shaanxi,Guangzhou,Huaihai and Henan regions(P<0.05).Conclusion There are differences in the distribution of CYP2C19 gene polymorphisms in CHD patients in the central and southern of Jiangsu,mainly with the medium and fast metabolic type,and there is no significant difference between genders,which can help the precise medication for the patients with coronary heart disease.