摘要
Industrial expansion has led to environmental pollution by xenobiotic compounds like polycyclic aromatic hydrocarbons and monoaromatic hydrocarbons.Pseudomonas spp.have broad metabolic potential for degrading aromatic compounds.The objective of this study was to develop a“biological funneling”strategy based on genetic modification to convert complex aromatic compounds into cis,cis-muconate(ccMA)using Pseudomonas putida B6-2 and P.brassicacearum MPDS as biocatalysts.The engineered strains B6-2(B6-2ΔcatBΔsalC)and MPDS(MPDSΔsalC(pUCP18k-catA))thrived with biphenyl or naphthalene as the sole carbon source and produced ccMA,attaining molar conversions of 95.3%(ccMA/biphenyl)and 100%(ccMA/naphthalene).Under mixed substrates,B6-2ΔcatBΔsalC grew on biphenyl as a carbon source and transformed ccMA from non-growth substrates benzoate or salicylate to obtain higher product concentration.Inserting exogenous clusters like bedDC1C2AB and xylCMAB allowed B6-2 recombinant strains to convert benzene and toluene to ccMA.In mixed substrates,constructed consortia of engineered strains B6-2 and MPDS specialized in catabolism of biphenyl and naphthalene;the highest molar conversion rate of ccMA from mixed substrates was 85.2%when B6-2ΔcatBΔsalC was added after 24 h of MPDSΔsalC(pUCP18k-catA)incubation with biphenyl and naphthalene.This study provides worthwhile insights into efficient production of ccMA from aromatic hydrocarbons by reusing complex pollutants.
基金
the National Key R&D Program of China(2021YFA0909500)
National Natural Science Foundation of China(32000062,32030004 and U22A20444)
Shanghai Excellent Academic Leaders Program(20XD1421900).
