五岭龙胆化学成分及其抗炎活性研究

Chemical constituents and anti-inflammatory activity of Gentiana davidii Franch.

研究五岭龙胆(Gentiana davidii Franch.)的化学成分及其体外抗炎活性。综合应用硅胶柱色谱、反相ODS柱色谱和Sephadex LH-20凝胶柱色谱等分离技术,对五岭龙胆70%乙醇提取物的乙酸乙酯和正丁醇萃取部位进行系统分离纯化,并结合化合物理化性质、波谱数据及与文献数据比对等进行结构鉴定,从中共分离鉴定15个化合物,分别为熊果酸(1)、乌发醇(2)、ursaldehyde(3)、28-norurs-12-en-3β-ol(4)、齐墩果酸(5)、β-amyrin-n-nonyl ether(6)、vanillyl glycol(7)、β-谷甾醇(8)、β-胡萝卜苷(9)和anemarrhenoside B(10)、正二十六烷(11)、正三十一烷醇(12)、1,5-bisβ-D-glucopyranosyloxy-2-(3′,3′-dimethylallyl)benzene(13)、2,4-二叔丁基苯酚(14)和2,2′-oxybis(1,4-di-tert-butylbenzene)(15)。化合物7、14和15为龙胆属首次分离得到,所有化合物均为五岭龙胆首次分离得到。对化合物1~15进行抗炎活性筛选发现,与模型组比较,化合物1、5和9对脂多糖诱导的RAW 264.7细胞释放的NO有显著抑制作用。

This study aimed to investigate the chemical constituents and anti-inflammatory activity in vitro from Gentiana davidii Franch..The chemical constituents of the ethyl acetate and n-butanol fractions of 70%ethanol extract in G.davidii were separated and purified by silica gel,ODS gel,and Sephadex LH-20 gel column chromatographies.Their structures were identified by physicochemical properties,spectroscopic analysis,as well as comparisons with the data reported in literature.Fifteen compounds were isolated and identified as ursolic acid(1),uvaol(2),ursaldehyde(3),28-norurs-12-en-3β-ol(4),oleanolic acid(5),β-amyrin-n-nonyl ether(6),vanillyl glycol(7),β-sitosterol(8),β-daucosterol(9),anemarrhenoside B(10),n-hexacosane(11),n-hentriacontyl alcohol(12),1,5-bisβ-D-glucopyranosyloxy-2-(3′,3′-dimethylallyl)benzene(13),2,4-di-tert-butylphenol(14),2,2′-oxybis(1,4-di-tert-butylbenzene)(15).Compounds 7,14 and 15 were first isolated from Gentiana.All the compounds were first isolated from G.davidii.The compounds 1-15 were preliminarily examined for anti-inflammatory activity in vitro.Compared with the model group,compounds 1,5 and 9 significantly inhibited the production of NO in lipopolysaccharide(LPS)-stimulated RAW 264.7 cells.