氧化玉米淀粉-聚己内酯基抗菌膜的制备及性能

Preparation and properties of oxidized corn starch-based nonionic antibacterial films

通过简便的“一锅法”对氧化玉米淀粉(OCS)进行线性接枝吲哚-3-乙酸(IAA),成功制得了新型氧化玉米淀粉基抗菌材料(OI)。然后,将OI与聚己内酯(PCL)进行共混成膜,制得了氧化玉米淀粉-聚己内酯基抗菌膜(PO)。通过FTIR、UV-Vis-NIR分光光度计、XRD、视频光学接触角测量仪、电子拉力机对所制备的OI、PO的结构和性能进行了表征和测试,并评价了PO的抗菌性能和生物相容性。结果表明,IAA与OCS主要通过酯化反应进行结合,制备的OI的取代度为60%、最低抑菌浓度为0.50 g/L,远低于传统抗生素和大分子壳聚糖抗菌材料的用量。随着OI用量的增加,PO结晶度下降,透光率下降,接触角略有下降,而抗拉强度先增加后降低再增加。当m(PCL)∶m(OI)=4:1时,制备的PO-20表现出良好的非浸出性、生物相容性。PO-20对大肠杆菌和金黄色葡萄球菌的抑菌圈直径分别为(15.2±0.10)和(15.0±0.07)mm。

A new oxidized corn starch-based antimicrobial material(OI)was successfully prepared from linear grafting of 3-indoleacetic acid(IAA)on oxidized corn starch(OCS)via a simple"one-pot"method,and further blended with polycaprolactone(PCL)to synthesize oxidized corn starch-polycaprolactone based antimicrobial films(PO).The structure and properties of the OI and PO obtained were characterized and analyzed by FTIR,UV-Vis-NIR spectrophotometer,XRD,video optical contact angle measuring instrument,and electronic tensile machine,followed by evaluation on the antibacterial properties and biocompatibility of PO.The results showed that IAA was covalently grafted to OCS mainly through esterification reaction,and the obtained OI displayed a substitution degree of 60%with a minimum inhibitory concentration of 0.50 g/L,which was much lower than the dosage of traditional antibiotics and macromolecule chitosan antibacterial materials.With the increase of OI dosage,the PO exhibited decrease in crystallinity,transmittance and contact angle,while the tensile strength first increased,then decreased and then increased.When m(PCL)∶m(OI)=4∶1,the prepared PO-20 showed good non-leachability,biocompatibility,and the inhibition zone diameters of PO-20 on Escherichia coli and Staphylococcus aureus were(15.2±0.10)mm and(15.0±0.07)mm,respectively.