基于MAGIC群体瓠瓜白粉病抗性的全基因组关联分析

Genome wide association analysis of powdery mildew resistance of bottle gourd based on MAGIC population

白粉病是葫芦科作物共有的病害之一,严重影响瓠瓜的产量和品质。本研究采用8个瓠瓜亲本F 8代的203个MAGIC(multi-parent advanced generation inter-cross)群体,利用2020和2021年两年的抗病表型数据,基于基因重测序过滤筛选得到的221043个高质量SNPs进行全基因组关联分析(genome-wide association study,GWAS)。在两年数据中重复检测到46个SNPs位点与白粉病抗性显著相关,在与抗病相关联的候选区段内共检测到32个候选基因,其中有12个基因预测与瓠瓜抗病功能相关。本研究首次在葫芦科作物中构建了MAGIC群体,并通过GWAS分析进行白粉病抗病基因定位,为克隆瓠瓜抗白粉病相关基因奠定了基础,也对深入了解白粉病抗性机制、辅助培育抗病品种具有重要意义。

Powdery mildew is one of the common diseases of Cucurbitaceae crops,which seriously affects the yield and quality of bottle gourd.In this study,203 MAGIC(multi-parent advanced generation inter cross)populations from the F 8 generation of 8 gourd parents were used to conduct genome wide association analysis(GWAS)based on 221043 high-quality SNPs screened by gene re-sequencing filter using the disease resistant phenotype data in 2020 and 2021.In two years data,46 SNPs were repeatedly detected to be significantly related to powdery mildew resistance,and 32 candidate genes were detected in the candidate regions associated with disease resistance,of which 12 genes were predicted to be related to the disease resistance function of bottle gourd.In this study,MAGIC population was constructed in Cucurbitaceae crops for the first time,and the powdery mildew resistance gene was mapped through GWAS analysis,which laid a foundation for cloning powdery mildew resistance related genes of bottle gourd,and also had important significance for in-depth understanding of powdery mildew resistance mechanism and assisting in breeding resistant varieties.