利用SELEX技术筛选针对寄生虫的核酸适配体研究进展

Progress in screening nucleic acid aptamers against parasites using SELEX technology

核酸适配体是人工合成的寡核苷酸序列,经指数富集的配体系统进化技术(SELEX)体外筛选获得。近年来,核酸适配体在生物医学领域的研究备受关注,因其能够与寄生虫、细菌、病毒等不同病原体的特定靶标进行高亲和力的特异性结合。该文综述了利用指数富集配体系统进化技术筛选针对寄生虫抗原的核酸适配体研究进展,介绍了指数富集配体系统进化技术的基本原理和流程,并重点探讨了在寄生虫研究领域中应用该技术筛选核酸适配体的最新研究进展。

Nucleic acid aptamers are artificially synthesized oligonucleotide sequences obtained by in vitro screening with systematic evolution of ligands by exponential enrichment(SELEX).In recent years,nucleic acid aptamers have attracted great attention in biomedical research because of their ability to bind with high affinity to specific targets of different pathogens such as parasites,bacteria,and viruses.This paper reviews the progress of research on screening aptamers against parasite antigens using systematic evolution of ligands by exponential enrichment,introduces the basic principles and process of SELEX technology,and then discusses the latest research progress on the application of SELEX technology for screening aptamers in the field of parasite research.