UPLC-PDA联合UPLC-MS/MS法确证一批标称杨树花口服液中非法添加物甘草酸

UPLC-PDA Combined UPLC-MS/MS Method to Confirm the Illegal Addition of Glycyrrhizic Acid in One Batch of Nominal Yangshuhua Koufuye

采用UPLC-PDA联合UPLC-MS/MS方法确证了一批标称杨树花口服液中非法添加物甘草酸。样品经提取稀释后,采用ACQUITY UPLC T3色谱柱为分离柱,UPLC-PDA初步筛查,再用负离子扫描,液相色谱串联质谱仪上机测定。通过对比色谱图保留时间和光谱图的峰形以及质谱图,结果表明最终确证该批样品中非法添加物为甘草酸。UPLC-PDA方法中甘草酸检测限为20μg/mL,定量限为50μg/mL。本方法快速、灵敏、重现性好,适用于本批标称杨树花口服液中甘草酸的检测。

The illegal addition of glycyrrhizic acid in a batch of nominal Yangshuhua Koufuye was confirmed using UPLC-PDA combined with UPLC-MS/MS method.After extraction and dilution,ACQUITY UPLC T3 column was used as separation column,UPLC-PDA,and liquid chromatography tandem mass spectrometer.By comparing the retention time of the chromatogram and the peak shape of the spectrogram and the mass spectrometer,the results showed that the illegal addition was glycyrrhizic acid.For UPLC-PDA,the limit of detection was 20μg/mL and the limit of quantification was 50μg/mL.This method is fast,sensitive and reproducible,and is suitable for the detection of glycyrrhizic acid in this nominal Yangshuhua koufuye.