2022年广西壮族自治区玉林市食品及公共场所从业人员沙门菌携带状况及耐药分析

Carriage and drug resistance of Salmonella in employees engaged in food and public place services in Yulin,Guangxi Zhuang Autonomous Region,2022

目的了解广西壮族自治区玉林市食品及公共场所行业从业人员沙门菌携带情况、菌型分布及耐药性,为制订玉林市肠道传染病防治规划提供依据。方法对玉林市食品及公共场所行业从业人员肛拭子进行沙门菌分离培养及血清分型;采用微量肉汤稀释法对沙门菌进行28种药物敏感试验。结果共检测标本54588份,沙门菌总检出率为1.85%(1012株),夏秋季高于冬春季。1012株沙门菌随机用了2种药敏板(905株沙门菌用NMIC-413药敏板,107株沙门菌用了RUONMIC-801药敏板)。因抗生素种类有差异,无法进行统计分析,现分析905株NMIC-413药敏板的结果。1012株沙门菌分为56个血清型,前3位血清型分别是鼠伤寒沙门菌(22.83%)、里森沙门菌(11.07%)和阿贡纳沙门菌(6.03%)。905株菌药敏检测结果显示,总耐药率为68.84%,菌株对四环素耐药率最高,达59.34%,次之为氯霉素(49.28%)和复方新诺明(37.13%)。多重耐药率为40.55%。结论玉林市食品、公共场所从业人员沙门菌携带率较高且血清型分布较广;沙门菌检出率具有季节性,菌株的耐药水平较高且多重耐药较严重。为此,应加强健康监测,及早发现沙门菌携带者并进行规范服药。

Objective To understand the carriage,bacterial type distribution,and drug resistance of Salmonella in employees in the food and public place services in Yulin,Guangxi Zhuang Autonomous Region,and provide evidence for the development of local prevention and control plan for intestinal infectious diseases.Methods Salmonella isolation and serotyping were performed by using anal swabs collected from the employees;Drug susceptibility of the Salmonella strains to 28 antibiotics were tested by using micro broth dilution method.Results A total of 54588 samples were tested,and the detection rate of Salmonella was 1.85%(1012/54588).The detection rate was higher in summer and autumn than winter and spring.Two drug sensitive plates were randomly used for 1012 Salmonella strains(NMIC-413 drug sensitive plate for 905 Salmonella strains,and RUONMIC-801 drug sensitive plate for 107 Salmonella strains).Due to the difference of antibiotic types,statistical analysis could not be carried out,only analyze the results of 905 strains of NMIC-413 drug sensitive plates.The 1012 strains of Salmonella were divided into 56 serotypes.The first three serotypes were S.typhimurium(22.83%),S.lissenii(11.07%)and S.argonne(6.03%).The drug susceptibility test results of 905 strains showed that the drug resistance rate was 68.84%,and the resistance rate of strains to tetracycline was highest(59.34%),followed by that to chloramphenicol(49.28%)and trimethoprim/sulfamethoxazole(37.13%).The multidrug resistance rate was 40.55%.Conclusion The Salmonella carrying rate was high in the employees engaged in food and public place services in Yulin,and the serotypes of the Salmonella detected were complex.The detection rate of Salmonella varied with season,and the drug resistance level of the strains was high and multiple drug resistance was serious.It is necessary to strengthen the health surveillance for the early detection of Salmonella carriers and standardized medication.