金霉素菌渣废弃物降解菌剂的筛选及堆肥预发酵研究

Screening of Degrading Bacteria for Chlortetracycline Extracted Residue and Compost Pre-fermentation Research

为了筛选金霉素降解菌并建立金霉素菌渣废弃物堆肥预发酵工艺,利用液体培养法筛选对金霉素耐受及降解的菌株,通过考察温度、pH、麸皮、移种量及时间对混合菌株发酵降解金霉素菌渣废弃物的影响,逐步建立固态发酵工艺,并在20 L的发酵罐进行验证。结果发现纳豆芽孢杆菌和黑曲霉对金霉素耐受且有不同程度的降解作用,两菌复合后,对添加10%麸皮的含1 mg·g^(-1)金霉素的pH 7为菌渣废弃物在20 L的发酵罐进行发酵,37℃条件下,发酵7 d后金霉素降解率达99%以上,达到无害化效果,物料干燥疏松,臭味少,为金霉素菌渣废弃物继续腐熟并实现资源化利用提供科学的依据。

To screen chlortetracycline degradation bacteria and establish the composting pre-fermentation process of chlortetracycline extracted residue,strains resistant and degrading to chlortetracycline were screened by liquid culture method,the effects of temperature,pH,wheat bran,inoculation quantity and time on the degradation of chlortetracycline extracted residue by mixed strain fermentation were investigated,and the solid-state fermentation process was established gradually and validated in a 20 L fermentor.The results showed that Bacillus natto and Aspergillus niger were resistant to chlortetracycline and could degrade chlortetracycline to different degrees.The mixed strains were inoculated to ferment the residue waste containing 1 mg·g^(-1) of chlortetracycline with 10%wheat bran and pH 7 in a 20 L fermenter.After 7 days fermentation under the condition of 37℃,the degradation rate of chlortetracycline was over 99%,which achieved harmless effect and the material was dry and loose,with less odor.It provides a scientific basis for the utilization of aureomycin residue waste by continuing decompose and mature as a resource.