八角枫药材质量控制含量测定方法研究

Determination method of total alkaloids in Alangium chinense

目的:建立八角枫总生物碱含量测定方法,测定黔产八角枫中的水分、浸出物含量,并规定其限度范围,为八角枫质量控制提供实验依据。方法:以八角枫碱为标准品,采用酸性染料比色法测定八角枫中总生物碱的含量,依照《中国药典》2020年版水分和浸出物测定方法,测定八角枫中水分和浸出物含量。结果:八角枫碱在0.180~0.450 mg/mL浓度范围内,回归方程Y=1.6024X-0.0389,R~2=0.9996,吸光度与浓度具有良好线性关系,平均加样回收率为100.19%,RSD<3%。不同产地10批次八角枫药材总生物碱含量在0.015%~0.029%之间,水分含量在4.36%~9.00%之间,水溶性浸出物含量在10.23%~24.59%之间,醇溶性浸出物含量在9.69%~23.81%之间,建议水分含量不得超过8.50%,水溶性浸出物含量和醇溶性浸出物分别不得低于10.06%和9.54%。结论:采用酸性燃料比色法测定八角枫中的总生物碱方法操作简单、重复性好、准确度高,同时,水分和浸出物含量的测定结果可以用于八角枫药材的质量控制。

In this study we established a method for the determination of total alkaloids in Alangium chinense.The contents of water and extracts in A.chinense from Guizhou were determined,and their limit ranges were specified,so as to provide experimental basis for the quality control of A.chinense.Using L(-)Anabasinein as the control substance,the content of total alkaloids in A.chinense was determined by acid dye colorimetric method.According to the determination methods provided in the Chinese Pharmacopoeia 2020,the contents of water and extractsin A.chinense were determined.In the concentration range of 0.180 mg/mL to 0.450 mg/mL,the regression equation was Y=1.6024X-0.0389,R~2=0.9996,the absorbance and concentration had a good linear relationship,the average recovery was 100.19%,RSD3%.The total alkaloidsin 10 batches of A.chinense from different origins was between 0.015% and 0.029%,the water content was between 4.36% and 9.00%,the content of water-soluble extracts was between 10.23% and 24.59%,and the content of alcohol-soluble extracts was between 9.69% and 23.81%.It is recommended that the water content should not exceed 8.50%,and the content of water-soluble extracts and alcohol-soluble extracts should not be less than 10.06% and 9.54%,respectively.The method for the determination of total alkaloids in A.chinense is simple to operate,with good accuracy and reproducibility.The determination results of water content and extracts can be used for the quality control of A.chinense.