结直肠癌患者外周血淋巴细胞亚群比例及表面受体表达的变化

Changes in the proportion of lymphocyte subsets and the expression of surface.receptors in peripheral blood of patients with colorectal cancer

目的 根据结直肠癌T细胞、自然杀伤(NK)细胞、 NKT细胞表面蛋白受体表达情况,探索结直肠癌患者免疫功能评价体系。方法 收集144例结直肠癌患者和87例健康对照组的外周血样本,通过流式细胞术、细胞培养等方法,分析患者外周血淋巴细胞亚群表面受体与健康对照组的差异。结果 与健康对照组相比,结直肠癌患者外周血总淋巴细胞、 CD16^(bright)CD56^(dimN)K细胞、 NKT细胞百分比降低;T细胞、 CD16^(bright)CD56^(dim)NK细胞、 NKT细胞表面抑制型受体T细胞免疫球蛋白和免疫受体酪氨酸抑制基序结构域(TIGIT)百分比增加;T细胞、 NK细胞、 NKT细胞表面趋化因子受体CC趋化因子受体7(CCR7)略降低。结论 结直肠癌患者外周血NK细胞亚群比例及表面受体表达谱存在差异。

ObjectiveTo identify the sets of lymphocytes that could systematically evaluate immune function of colorectal cancer patients,based on the expression of colorectal cancer T cells,natural killer(NK)cells,and NKT cell surface protein receptors.Methods Peripheral blood samples from 144 patients with colorectal cancer and 87 healthy controls were collected,and the differences in surface receptors of lymphocyte subsets in peripheral blood of patients and healthy controls were analyzed by means of flow cytometry and cell culture.Results Compared with healthy control group,the percentage of peripheral blood total lymphocytes,CD16^(bright) CD56^(dim) NK cells and NKT cells decreased in patients with colorectal cancer.The percentage of T cells,CD16^(bright) CD56^(dim) NK cells and NKT cell surface inhibitory receptors T-cllimmunoglobulin and immunoreceptor tyrosine-based inhibitor motif domains(TIGIT)increased;T cells,NK cells,NKT cell surface chemokine receptor C-C motif chemokine receptor 7(CCR7)slightly decreased.Conclusion There are differences in the proportion of NK cell subsets and the expression profile of surface receptors in peripheral blood of patients with colorectal cancer.