慢性阻塞性肺病肠道和肺炎性2型固有淋巴样细胞(iILC2)及相关细胞因子含量分析

Intestinal and lung inflammatory group 2 innate lymphoid cells(iILC2s)and their related cytokines in chronic obstructive pulmonary disease

目的 探讨慢阻塞性肺病(COPD)肠道炎性2型固有淋巴细胞(iILC2)与肺部ILC2及其相关炎症反应的关系。方法采用熏烟法建立COPD小鼠模型,将小鼠随机分为正常组和COPD组。采用HE染色检测小鼠肺、肠组织的病变情况,流式细胞术检测天然ILC2细胞(nILC2)和iILC2细胞数量。使用Wright-Giemsa染色检测正常组和COPD组小鼠支气管肺泡灌洗液(BALF)中免疫细胞数量,ELISA检测BALF中白细胞介素13(IL-13)和IL-4的含量。结果 COPD小鼠肺、肠组织上皮细胞增生,部分萎缩或缺失,伴随炎细胞浸润,病理评分增加,BALF中中性粒细胞、单核细胞、淋巴细胞显著增加。COPD小鼠肺组织iILC2数量、肠组织nILC2和iILC2数量显著增加,BALF中IL-13和IL-4含量显著升高。结论 COPD肺部iILC2及其相关细胞因子的增加可能与肠道iILC2细胞相关。

Objective To investigate the relationship between intestinal inflammatory group 2 innate lymphoid cells(ilLC2s)and lung ILC2s and its inflammatory response in chronic obstructive pulmonary disease(COPD).Methods Mouse COPD model was established by smoking method.The mice were randomly divided into normal group and COPD group.HE staining was used to detect the pathological changes in lung and intestine tissues of mice in normal group and COPD group,and the contents of natural ILC2s(nlLC2s)and ilLC2s cells were measured by flow cytometry.Wright-Giemsa staining was used to measure the number of immune cells in the bronchoalveolar lavage fluid(BALF)of mice in normal group and COPD group,and the concentration of IL-13 and IL-4 was detected by ELISA.Results In COPD mice,epithelial cells of the lung and intestinal tissues exhibited pathological hyperplasia,partial atrophy or deletion,inflammatory cell infitration,increased pathological score and significantly increased neutrophils,monocytes,and lymphocytes in BALF.Lung ilLC2s,intestinal nILC2s and ilLC2s were increased significantly in the COPD group.The contents of IL-13 and IL-4 in BALF were significantly increased.Conclusion The increase of ilLC2s and their related cytokines in COPD lung may be related to intestinal inflammatory ILC2s.