微囊藻毒素亮氨酸精氨酸(MC-LR)损伤睾丸支持细胞免疫反应及细胞生物学行为的机制研究进展

The mechanism of microcystin leucine-arginine(MC-LR)-induced injury of Sertoli cell immune response and biological behavior

微囊藻毒素亮氨酸精氨酸(MC-LR)是水华爆发时微囊藻属、鱼腥藻属等蓝藻产生的一种对人类有致癌性的藻毒素。MC-LR可对雄性动物生殖系统产生毒性,导致不育,主要表现为诱导细胞凋亡、破坏细胞骨架、干扰DNA损伤修复途径或损伤血睾屏障(BTB)功能等。睾丸支持细胞(Sertoli细胞)是生精小管中与生精细胞直接接触的体细胞,可通过分泌多种细胞因子和免疫抑制因子调节免疫反应以维持睾丸免疫稳态,亦可与邻近生殖细胞形成BTB,为各级生精细胞提供营养、支持和保护作用的微环境。MC-LR可引发睾丸Sertoli细胞炎症、诱导细胞凋亡、破坏BTB完整性,进而造成睾丸生精功能障碍。

Microcystin-leucine arginine(MC-LR),a potentially carcinogenic toxin,is produced by Cyanobacteria such as Microcystis and Ananabacteria during water bloom.Increasing evidence demonstrated that MC-LR induces male reproductive toxicity,mainly by inducing germ cell apoptosis,destroying cell cytoskeleton,interfering with DNA damage repair pathway,and damaging blood-testicular barrier(BTB),which eventually lead to male sterilty.Testicular Sertoli cells are the somatic cells that directly contact with spermatogenic cells in seminiferous tubules.They not only regulate immune response to maintain testicular immune homeostasis by secreting a variety of cytokines and immunosuppressive factors,but also provide the protective effects of spermatogenic cells by forming BTB.MC-LR induces inflammation and apoptosis of Sertoli cells,and destroys the integrity of the BTB,and then causes spermatogenesis dysfunction.