摘要
目的:探讨沉默TCP1α蛋白对人胰腺导管腺癌细胞的影响。方法:采用SiRNA干扰技术沉默两种人胰腺癌细胞(PANC-1和Mia Pa Ca-2)中TCP1α蛋白基因,Western blot检测3种TCP1α蛋白(TCP1α-1、TCP1α-2、TCP1α-3)表达情况,验证SiRNA沉默效果。以体外增殖实验(MTT实验)和Transwell迁移侵袭实验验证抑制TCP1α蛋白表达对两种胰腺癌细胞体外增殖、迁移及侵袭等生物学行为的影响。Western blot检测PD98059和CHX共同处理的基因沉默人胰腺癌细胞的TCPIα蛋白表达情况。结果:沉默TCP1α基因后两种人胰腺癌细胞增殖和迁移侵袭能力显著降低。结论:抑制TCP1α蛋白表达能明显降低人胰腺导管腺癌细胞的增殖侵袭及迁移能力,且能诱发人胰腺导管腺癌细胞的急性凋亡。
Objective:To clarify the role of TCP1αon the occurrence and development of human pancreatic ductal adenocarcinoma,and to provide new ideas for the treatment.Methods:Small interfering RNA silenced the expression of TCP1αin human pancreatic cancer cells(MiaPaCa-2 and PANC-1),and the effect of different expression levels of TCP1αon pancreatic cancer was detected by proliferation assay(MTT method)and transwell migration and invasion assay.in vitro.The cells silenced by TCP1αwere treated with PD98059(40μmol/L)and CHX(10μg/mL),and the expression of downstream proteins was detected by Western Blot assay.Result:The proliferation assay(MTT method),migration and invasion assay found that MiaPaCa-2 and PANC-1 with low expression of TCP1αwere significantly reduced both in eproliferation,migration and invasion abilities.After treatment with PD98059 and CHX,the expression level of apoptosis protein PARP was significantly decreased.Conclusion:Inhibiting the expression of TCP1αcan significantly reduce the proliferation,invasion and migration ability of pancreatic cancer cells,and partially induce the acute apoptosis of pancreatic cancer cells.
作者
王乐乐
赵林林
亓同刚
WANG Le-le;ZHAO Lin-lin;QI Tong-gang(Laboratory Medical Center,The Second Hospital of Shandong University,Jinan 250033,China;Central Research Laboratory,Liao Cheng People,s Hospital,LiaoCheng 252000,China)
出处
《中国现代普通外科进展》
CAS
2023年第10期762-766,共5页
Chinese Journal of Current Advances in General Surgery