沉默TCP1α蛋白对人胰腺导管腺癌细胞影响的研究

Study of the effect of Silencing TCP1αon human pancreatic ductal adenocarcinom

目的:探讨沉默TCP1α蛋白对人胰腺导管腺癌细胞的影响。方法:采用SiRNA干扰技术沉默两种人胰腺癌细胞(PANC-1和Mia Pa Ca-2)中TCP1α蛋白基因,Western blot检测3种TCP1α蛋白(TCP1α-1、TCP1α-2、TCP1α-3)表达情况,验证SiRNA沉默效果。以体外增殖实验(MTT实验)和Transwell迁移侵袭实验验证抑制TCP1α蛋白表达对两种胰腺癌细胞体外增殖、迁移及侵袭等生物学行为的影响。Western blot检测PD98059和CHX共同处理的基因沉默人胰腺癌细胞的TCPIα蛋白表达情况。结果:沉默TCP1α基因后两种人胰腺癌细胞增殖和迁移侵袭能力显著降低。结论:抑制TCP1α蛋白表达能明显降低人胰腺导管腺癌细胞的增殖侵袭及迁移能力,且能诱发人胰腺导管腺癌细胞的急性凋亡。

Objective:To clarify the role of TCP1αon the occurrence and development of human pancreatic ductal adenocarcinoma,and to provide new ideas for the treatment.Methods:Small interfering RNA silenced the expression of TCP1αin human pancreatic cancer cells(MiaPaCa-2 and PANC-1),and the effect of different expression levels of TCP1αon pancreatic cancer was detected by proliferation assay(MTT method)and transwell migration and invasion assay.in vitro.The cells silenced by TCP1αwere treated with PD98059(40μmol/L)and CHX(10μg/mL),and the expression of downstream proteins was detected by Western Blot assay.Result:The proliferation assay(MTT method),migration and invasion assay found that MiaPaCa-2 and PANC-1 with low expression of TCP1αwere significantly reduced both in eproliferation,migration and invasion abilities.After treatment with PD98059 and CHX,the expression level of apoptosis protein PARP was significantly decreased.Conclusion:Inhibiting the expression of TCP1αcan significantly reduce the proliferation,invasion and migration ability of pancreatic cancer cells,and partially induce the acute apoptosis of pancreatic cancer cells.