陈皮多糖片段的体外益生活性分析

In Vitro Prebiotic Activity of Polysaccharide Fractions from Tangerine Peel

以10年份陈皮水溶性多糖Cpp为研究对象,采用酸解、离子交换吸附和凝胶排阻色谱分离得到3种多糖片段(f1-1、f2-2和f3-2),比较它们的结构和益生活性。结果表明,片段f2-2分子质量最小,为1.15×10^(4)Da,主要由鼠李糖、阿拉伯糖和半乳糖组成,物质的量比为0.21∶1.00∶0.25。体外益生活性实验显示多糖片段f2-2的添加促进了乳酸杆菌和双歧杆菌的增殖,乳酸杆菌和双歧杆菌数量分别为对照组(添加1 mg/mL葡萄糖)的3.2倍和2.9倍(P<0.05);与对照组相比,Cpp的添加仅对乳酸杆菌增殖有显著促进作用(P<0.05)。与对照组相比,片段f2-2的添加显著提高了粪便发酵上清液中丙酸的产量(P<0.05);此外,片段f1-1或f2-2添加均促进了丁酸的产生。综上,10年份陈皮多糖片段呈现出显著的益生元特征,其活性与片段结构联系密切。

Crude water-soluble polysaccharides(Cpp)from 10-year-old tangerine peel were hydrolyzed with sulphuric acid and fractionated by successive ion exchange adsorption and size exclusion chromatography into three major fractions(f1-1,f2-2 and f3-2).The structures and prebiotic activities of Cpp and the fractions were compared.The results showed that f2-2 had the smallest molecular mass of 1.15×10^(4)Da and was mainly composed of rhamnose,arabinose and galactose with a molar ratio of 0.21:1.00:0.25.f2-2 could promote the proliferation of Lactobacilli and Bifidobacterium,increasing their counts 3.2 and 2.9 times compared with the control group(with 1 mg/mL glucose),respectively.Cpp could only promote the proliferation of Lactobacilli significantly(P<0.05).Compared with the control group,the addition of f2-2 significantly increased the production of propionic acid in the culture supernatant of human fecal bacteria(P<0.05).Moreover,the addition of f1-1 or f2-2 promoted the production of butyric acid.To sum up,the polysaccharides from 10-year-old tangerine peel showed significant prebiotic characteristics,which had a close relationship the structures of the polysaccharides.