四川省绵阳市猪细小病毒2型病原检测及遗传变异分析

Pathogenic Detection and Genetic Variation Analysis of Porcine Parvovirus Type 2 in Mianyang City,Sichuan Province

猪细小病毒2型(porcine parvovirus type 2,PPV2)是2001年首次在缅甸发现的新型病毒。为深入了解四川省绵阳市PPV2感染及遗传变异情况,使用普通PCR对采自4个地区10个规模化猪场的296份临床病猪血清样本进行了核酸检测,并对其中1份阳性样品进行了全基因组测序和遗传变异分析。结果显示:PPV2检出率为1.69%(5/296);阳性样品(PPV2-11-MY)全基因组长度为5506 bp,与12个PPV2参考毒株的核苷酸同源性为93.96%~99.30%,氨基酸同源性为52.50%~73.80%;存在3处氨基酸高变区域,分别位于91~218 aa、513~626 aa和776~1065 aa;该毒株同源性最接近我国江苏省毒株MW853949.1。本研究不仅丰富了PPV2流行病学数据,为后续疫苗研究提供了理论依据,同时也为进一步研究PPV2致病机理及毒力奠定了基础。

Porcine parvovirus type 2(PPV2)was a novel virus that was firstly found in Myanmar in 2001.In order to further investigate the infection and genetic variation of PPV in Mianyang City,Sichuan Province,296 serum samples collected from the clinical diseased pigs in 10 intensive farms across 4 regions were for nucleic acid detection by PCR,followed by whole genome sequencing and genetic variation analysis based on one of positive samples.The results revealed that the detection rate of PPV2 was 1.69%(5/296);the whole genome length of the positive sample(PPV2-11-MY strain)was 5506 bp,the amino acid homology was from 52.50%to 73.80%compared to 12 PPV2 reference strains,and the nucleotide homology was from 93.96%to 99.30%,with 3 amino acid hypervariable regions locating at 91-218 aa,513-626 aa and 776-1065 aa,respectively;and the strain was closest to the MW853949.1 isolated in Jiangsu Province,China.The study not only enriched the epidemiological data about PPV2 that served as a theoretical basis for subsequent vaccine researches,but also laid a foundation for further researches on the pathogenesis and virulence of the virus.