S100A6对肺鳞癌细胞株H520生物学行为的影响研究

Effect of S100A6 on Biological Behavior of Lung Squamous Cell Strain H520

目的 分析S100A6对肺鳞癌细胞株H520生物学行为的影响。方法 本实验时间为2021年11月至2022年11月。取对数生长期的H520,将其随机分为H520组、H520+空载体组、H520+S100A6组,其中H520组不进行干预,H520+空载体组转染空载体质粒,H520+S100A6组转染过表达S100A6的载体质粒。采用细胞计数试剂盒(CCK-8)检测各组细胞增殖率,采用细胞划痕试验检测各组细胞迁移距离,采用Transwell实验检测各组细胞侵袭数,采用流式细胞术检测各组细胞周期。结果 H520+S100A6组48、72 h时细胞增殖率高于H520组、H520+空载体组(P<0.05)。H520+S100A6组细胞迁移距离长于H520组、H520+空载体组(P<0.05)。H520+S100A6组细胞侵袭数多于H520组、H520+空载体组(P<0.05)。H520+S100A6组G1期细胞占比高于H520组、H520+空载体组,G2期细胞占比低于H520组、H520+空载体组(P<0.05)。结论 S100A6可促进肺鳞癌细胞株H520增殖、迁移、侵袭,促使细胞进入分裂周期。

Objective To analyze the effect of S100A6 on biological behavior of lung squamous cell strain H520.Methods This study was conducted from November 2021 to November 2022.H520 at logarithmic growth stage was randomly divided into H520 group,H520+empty carrier group and H520+S100A6 group,in which H520 group did not receive intervention,H520+empty carrier group was transfected with empty plasmid and H520+S100A6 group was transfected with carrier plasmid overexpressing S100A6.Cell proliferation rate was detected by cell counting kit-8(CCK-8),cell migration distance was detected by cell scratch assay,cell invasion number was detected by Transwell assay,and cell cycle was detected by flow cytometry.Results At 48 and 72 hours,the cell proliferation rate of H520+S100A6 group was higher than that of H520 group and H520+empty carrier group(P<0.05).The cell migration distance of H520+S100A6 group was longer than that of H520 group and H520+empty carrier group(P<0.05).The number of cell invasion in H520+S100A6 group was higher than that in H520 group and H520+empty carrier group(P<0.05).The proportion of G_(1) phase cells in H520+S100A6 group was higher than that in H520 group and H520+empty carrier group,and the proportion of G_(2) phase cells was lower than that in H520 group and H520+empty carrier group(P<0.05).Conclusion S100A6 can promote the proliferation,migration and invasion of lung squamous cell strain H520,and promote the cell to enter the division cycle.