基于点源成像的微球成像分辨能力仿真分析

Simulation Analysis of Microsphere Imaging Resolution Ability Based on Point Source Imaging

基于微球透镜的显微成像技术具有方法简单、无需标记等优点,近年来受到了国内外众多研究者的关注。微球透镜成像系统参数变化将直接影响分辨能力,然而,目前缺乏此方面的仿真分析工作。因此,基于点源成像,提出了一种分析微球透镜成像分辨能力的方法,仿真分析了微球尺寸以及微球和点光源距离变化对成像分辨能力的影响。结果表明,成像分辨能力随微球尺寸的增大而下降,微球和点光源距离小于800 nm时,分辨能力随距离的增加呈下降趋势,当距离大于800 nm时,分辨能力不再发生明显变化。仿真结果对微球透镜显微成像实验具有指导意义,提出的方法为仿真分析微球成像分辨能力的研究工作提供了理论基础。

The microscopic imaging technology based on microspheres has been popular in recent years due to its advantages of being simple and free-labeled.However,there is a lack of analytical research on how imaging parameters influence imaging resolution ability.A method for analyzing the imaging resolution ability based on the point source imaging is proposed.The influence of the microsphere size,the distance between the microsphere and the point source on the imaging resolution ability is analyzed by simulations.The results demonstrate that the imaging resolution ability decreases with the increase of the microsphere size.The imaging resolution ability also shows a downward trend with the increase of the distance between the microsphere and the point source when the distance is less than 800 nm.When the distance is more than 800 nm,the imaging resolution ability doesn’t decrease significantly.The simulation results could guide experiments based on microspheres.The proposed method provides the theoretical basis for the research work on the simulation analysis of the imaging resolution ability of microspheres.