miRNA在哮喘患儿呼出气冷凝物中的表达水平及诊断价值

Expression of miRNA in exhaled condensation in children with asthma and its diagnostic value

目的探讨呼出气冷凝物(EBC)中微小RNA(miRNA)作为诊断哮喘患儿生物标志物的诊断价值。方法选取2021年3月至2022年12月华北石油管理局总医院收治的7~12岁哮喘患儿80例作为哮喘组,并选取同期同年龄段的健康志愿者80例作为对照组。检测2组受试者EBC中11种相关miRNA的表达水平。应用主成分分析法和因子分析法对miRNAs进行聚类分析。采用受试者工作特征(ROC)曲线的曲线下面积(AUC)分析miR-133-3p、miR-328-3p、miR-21-5p、miR-155-5p在哮喘患儿中的诊断意义。结果哮喘组EBC中miR-21-5p和miR-133-3p的相对表达水平低于对照组,差异有统计学意义(P<0.05),而miR-328-3p和miR-155-5p的相对表达水平高于对照组,差异有统计学意义(P<0.05)。根据对照组和哮喘组EBC中miR-133-3p、miR-328-3p、miR-21-5p、miR-155-5p的相对表达水平可以形成两个独立亚群,即体检健康者和哮喘患儿。因子分析法发现miR-21-5p、miR-155-5p可以聚成一个亚类(因子载荷分别为0.93和0.90),miR-133-3p、miR-328-3p聚成一个亚类(因子载荷分别为0.86和0.80)。哮喘患儿与健康儿童的miR-133-3p、miR-328-3p、miR-21-5p、miR-155-5p的灵敏度分别为93.77%、92.41%、95.23%、92.57%,特异度分别为94.01%、91.07%、91.48%、91.35%。miR-133-3p、miR-328-3p、miR-21-5p、miR-155-5p联合对哮喘患儿的诊断灵敏度为97.50%,特异度为92.50%,准确度为95.00%。结论EBC中miR-133-3p、miR-328-3p、miR-21-5p、miR-155-5p可作为诊断哮喘患儿的潜在标志。

Objective To explore the diagnostic value of miRNA in exhaled breath condensate(EBC)as a biomarker for the diagnosis of asthma in children.Methods A total of 80 children with asthma aged 7-12 in General Hospital of North China Petroleum Administration Bureau from March 2021 to December 2022 were selected as the asthma group,and 80 healthy volunteers of the same age were selected as the control group during the same period.The expression levels of 11 related miRNA in EBC of the two groups were detected.Principal component analysis and factor analysis were used to cluster miRNAs.The AUC of receiver operating characteristic curve(ROC)was used to analyze the diagnostic significance of miR-133-3p,miR-328-3p,miR-21-5p and miR-155-5p in children with asthma.Results The relative expression levels of miR-21-5p and miR-133-3p in EBC of asthma group were lower than those of control group,the differences were statistically significant(P<0.05),while the relative expression levels of miR-328-3p and miR-155-5p were higher than those of control group,the differences were statistically significant(P<0.05).According to the relative expression levels of miR-133-3p,miR-328-3p,miR-21-5p and miR-155-5p in EBC of subjects in the control group and asthma group,two independent subgroups could be formed,namely healthy physical examination subjects and children with asthma.Factor analysis showed that miR-21-5p and miR-155-5p could be clustered into a subclass(factor loading were 0.93 and 0.90,respectively),and miR-133-3p and miR-328-3p could be clustered into a subclass(factor loading were 0.86 and 0.80,respectively).The sensitivity of miR-133-3p,miR-328-3p,miR-21-5p and miR-155-5p in children with asthma and healthy children were 93.77%,92.41%,95.23%and 92.57%,respectively.The specificity were 94.01%,91.07%,91.48%and 91.35%,respectively.The diagnostic sensitivity,specificity and accuracy of miR-133-3p,miR-328-3p,miR-21-5p and miR-155-5p combined for children with asthma were 97.50%,92.50%and 95.00%,respectively.Conclusion miR-133-3p,miR-328-3p,miR-21-5p and miR-155-5p in EBC can be used as potential markers for the diagnosis of children with asthma.