副干酪乳酪杆菌甲基化转移酶突变体响应渗透胁迫的代谢组学研究

Metabolomic study of methylated transferase mutants of Lacticaseibacillus paracasei in response to osmotic stress

DNA甲基化是一种重要的表观遗传调控机制,与细菌的许多生理功能密切相关。该研究采用超高效液相色谱-四级杆飞行时间质谱技术,针对渗透胁迫环境比较分析了副干酪乳酪杆菌Zhang甲基化转移酶突变体及其野生型的代谢组,探究其显著差异代谢物质。通过进行主成分分析和正交偏最小二乘判别分析等多变量统计分析,同时结合数据库检索和质谱信息匹配,共筛选到10个显著差异代谢物(P<0.05)。这些代谢产物主要包括皮质醇、18-羟基皮质酮、脱氧肌苷、甜菜碱醛以及糖衣去氧胆酸等代谢物质,涉及到类固醇激素生物合成、嘌呤代谢、甘氨酸、丝氨酸和苏氨酸代谢以及初级胆汁酸代谢等多条代谢通路。该研究结果有助于揭示DNA甲基化介导副干酪乳酪杆菌Zhang适应渗透胁迫环境的分子机制。

DNA methylation is an important epigenetic regulatory mechanism,which is closely related to many physiological functions of bacteria.In this study,ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry was used to compare and analyze the Lacticaseibacillus paracasei Zhang methyltransferase mutant strain and its wild-type metabolome in an osmotic stress environment,and explore its significantly different metabolites.Through multivariate statistical analysis including principal component analysis and orthogonal partial least squares discriminant analysis,combined with database search and mass spectrometry information matching,a total of 10 significantly different metabolites were screened(P<0.05).These metabolites mainly include cortisol,18-hydroxycorticosterone,deoxyinosine,betaine aldehyde,and glycochenodeoxycholic acid,which were involved in multiple metabolic pathways such as steroid hormone biosynthesis,purine metabolism,glycine,serine,and threonine metabolism,and primary bile acid metabolism.These results help to reveal the molecular mechanism of DNA methylation mediating the adaptation of L.paracasei Zhang to osmotic stress environment.