泸州市与广州市龙眼核不同极性部位体外抗氧化活性的比较研究

Comparative study on antioxidative activity in vitro of different polar parts of longan kernels from Luzhou City and Guangzhou City

比较研究四川省泸州市和广东省广州市两个主产区的龙眼(Dimocarpus longan)核不同极性部位的体外抗氧化活性。以泸州张坝、泸州黄舣和广州荔湾产地的龙眼核为研究对象,经5种不同极性的溶剂提取其抗氧化活性物质,通过DPPH法和ABTS^(+)·法比较各提取物的抗氧化能力。结果表明,各极性部位3个产地(除泸州张坝外)龙眼核干膏质量的大小均表现为正丁醇>乙酸乙酯>无水乙醇>去离子水>石油醚。体外抗氧化试验结果表明,在DPPH法中,除广州荔湾产地龙眼核的石油醚部位外,其余产地的各极性部位均具有较高的自由基清除率,其中广州荔湾、泸州张坝产地乙酸乙酯部位的抗氧化能力最佳,IC_(50)分别为0.021、0.022 mg/mL。在ABTS^(+)·法中,各产地龙眼核的石油醚部位清除ABTS^(+)自由基能力最强、乙酸乙酯部位次之,且在乙酸乙酯提取部位中以泸州张坝龙眼核的抗氧化能力最佳,其IC_(50)为0.023 mg/mL。

The antioxidant activity in vitro of different polar parts of Longan(Dimocarpus longan)kernels produced in Luzhou City,Sichuan Province and Guangzhou City,Guangdong Province were studied.The antioxidant activity of longan kernels from Luzhou Zhangba,Luzhou Huangyi and Guangzhou Liwan were extracted by 5 solvents with different polarities.The antioxidant activity of extracts was compared by DPPH and ABTS^(+)·methods.The results showed that the weight of dry extracts of longan kernels from 3 producing areas(except Luzhou Zhangba)was n-butanol>ethyl acetate>anhydrous ethanol>deionized water>petroleum ether.The results of the in vitro antioxidant test showed that in DPPH method,except petroleum ether extract from longan kernels of Guangzhou Liwan producing area,all polar extract from other producing areas had higher free radical scavenging rate,and the ethyl acetate extract from Guangzhou Liwan producing area and Luzhou Zhangba producing area had the best antioxidant capacity,with IC_(50) of 0.021 mg/mL and 0.022 mg/mL,respectively.In ABTS^(+)·method,the petroleum ether extract of longan kernels from all producing areas had the strongest ability to remove ABTS^(+)free radicals,followed by ethyl acetate extract,and Luzhou Zhangba longan kernels extract had the best antioxidant ability,with IC_(50) of 0.023 mg/mL.