摘要
为探究长双歧杆菌A17胞外多糖(exopolysaccharides,EPS)生物合成途径,作者依托全基因组测序,利用eggNOG-mapper、KEGG、GO数据注释和局部序列比对等方法对EPS合成相关的基因进行注释与同源性分析。根据同源性分析得到一个长度约为20 kb由19个基因组成的A17 eps基因簇,预测到EPS前体糖核苷酸合成途径,同时发现该途径中的关键基因pgm并进行了异源表达。eps基因簇负责eps单体重复单元合成以及输出相关的EPS合成过程,在EPS前体糖核苷酸合成途径中双歧杆菌A17利用葡萄糖、乳糖形成UDP-Gal、UDP-Glc两种前体糖核苷酸。其中,pgm编码1→6葡萄糖磷酸变位酶,该酶的产量和活性与EPS合成有关。
To investigate the biosynthesis pathway ofexopolysaccharides(EPS)in Bifidobacterium longum A17,the authors relied on the whole-genome sequencing,and EPS synthesis-related genes were annotated and subjected to homology analysis using eggNOG-mapper,KEGG,GO data annotation,and local sequence alignments.According to the homology analysis,an A17 eps gene cluster consisting of 19 genes with a length of approximately 20 kb was obtained.The synthesis pathway of the precursor glyconucleotides of EPS was predicted,and the key gene pgm involved in the pathway was found and heterologously expressed.The eps gene cluster is responsible for the synthesis of eps monomer repeat units and the subsequent EPS synthesis process related to the export.In the EPS precursor glyconucleotide synthesis pathway,Bifidobacterium longum A17 utilizes glucose and lactose to form 2 precursor glyconucleotides,UDP-Gal and UDP-Glc.Among them,Pgm encodes 1-→6 glucose phosphomutase,whose production and activity are enzyme related to EPS synthesis.
作者
张文丽
王英
石莹
呼鑫荣
旭日花
ZHANG Wenli;WANG Ying;SHI Ying;HU Xinrong;XU Rihua(School of Life Sciences,Inner Mongolia University,Huhhot 010021,China)
出处
《食品与生物技术学报》
CAS
CSCD
北大核心
2023年第10期9-15,共7页
Journal of Food Science and Biotechnology
基金
国家自然科学基金项目(31860433)
内蒙古自治区自然科学基金项目(2018MS03026)。
关键词
双歧杆菌
胞外多糖
基因簇
异源表达
Bifidobacterium
exopolysaccharides
gene cluster
heterologous expression