恒温实时荧光法快速检测婴幼儿辅助食品中胡萝卜成分的研究

Study on Rapid Determination of Carrot Components in Complementary Foods for Infant and Young Children by Isothermal Real-Time Fluorescence Assay

为建立婴幼儿辅助食品中胡萝卜成分的恒温实时荧光快速检测方法,根据胡萝卜ITS1-5.8SRNA-ITS2基因序列设计合成恒温实时荧光法(LAMP)的特异性引物,以常见农产品及不同胡萝卜质量分数的白萝卜DNA分别验证方法的特异性和灵敏度,选取不同品系胡萝卜种子验证方法的适用范围,并通过预包装食品检测其实用性。结果表明:所建方法与其他物种无交叉反应,特异性强,能在1 h内检测低至质量分数为0.01%的胡萝卜成分,灵敏度高,适用于不同品系胡萝卜的检测。适用范围广,通过实际样本的检测验证,结果快速、准确。该方法操作简单、成本较低、数据智能判读实时同步、结果准确快速,可用于婴幼儿辅助食品中胡萝卜成分的快速检测,为婴幼儿食品标签管理及食品安全监管提供技术依据。

In order to establish a rapid detection method for carrot components in complementary foods for infant and young children using isothermal real-time fluorescence,specific primers for loop-mediated isothermal amplification(LAMP)were designed and synthesized based on the carrot ITS1-5.8 SRNA-ITS2 gene sequence.The specificity and sensitivity of the method were verified by DNA from common crop and white radish with different carrot mass ratios.Different cultivars of carrot seeds were selected to verify the applicability of the method,and its practicality was tested through pre-packaged food.The results indicated that the established method exhibited strong specificity without cross-reactivity with other species.It can detect the composition of carrot as low as 0.01%mass ratio within 1h,demonstrating high sensitivity.It is suitable for the detection of different carrot cultivars with a wide range of application.The method proved to be fast and accurate when validated with actual samples.This method offers simplicity,cost-effectiveness,real-time synchronization of intelligent data interpretation,and accurate and rapid visualization of results.Therefore,it can be used for the rapid detection of carrot components in complementary foods for infant and young children,providing a technical basis labeling management and food safety supervision in complementary foods for infant and young children.