2型糖尿病动脉粥样硬化患者血清FGF19与氧化应激的相关性研究

Relationship analysis between serum FGF19 and oxidative stress in type 2 diabetic patients with atherosclerosis

目的:观察2型糖尿病(type 2diabetes mellitus,T2DM)动脉粥样硬化(atherosclerosis,AS)患者血清中成纤维细胞生长因子(fibroblast growth factor,FGF)19水平,并探讨FGF19水平与氧化应激发生发展的相关性。方法:选取T2DM患者78例,以颈动脉内中膜厚度(carotid intima-media thickness,CIMT)为标准分为两组,T2DM患者(n=38)为对照组,T2DM合并AS患者(n=40)为实验组,记录所有入组患者血压、计算体质量指数(body mass index,BMI);检测血糖、血脂等相关生化指标;使用酶联吸附免疫法(ELISA)测定血清FGF19、丙二醛(malondialdehyde,MDA)、谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)、血红素氧合酶-1(heme oxygenase-1,HO-1)水平,生化法测定超氧化物过氧化歧化酶(superoxide peroxide dismutase,SOD)水平。观察两组之间相关指标水平差异,Pearson相关分析血清FGF19水平与HO-1、SOD、MDA、GSH-PX、CIMT、臂-踝脉冲波速(brachial ankle pulse wave velocity,baPWV)的相关性,多元线性回归分析CIMT的影响因素。结果:与对照组相比,实验组CIMT、baPWV、HO-1、MDA均升高(P<0.01),FGF19、SOD、GSH-Px明显降低(P<0.01)。相关性分析结果显示:实验组样本血清FGF19水平与HO-1、MDA、CIMT、baPWV呈负相关(r=-0.668、-0.657、-0.901、-0.734,P<0.01),与SOD、GSH-Px呈正相关(r=0.345、0.760,P<0.01)。多元线性回归分析表明FGF19、HO-1、SOD、MDA、GSH-PX均是CIMT的可能影响因素(P<0.05)。结论:T2DM合并AS患者血清FGF19的水平与氧化应激有一定关系,且对预测AS的发生及进展具有重要临床意义。

Objective:To investigate the relationship between serum fibroblasts growth factor(FGF)19 level and the development of atherosclerosis(AS)in patients with type 2 diabetes mellitus(T2DM).Methods:A total of 78 T2DM patients were selected and divided into two groups based on carotid intima-media thickness(CIMT).Among them,40 T2DM patients were control group and 38 T2DM patients with AS were experimental group.Record the blood pressure and body mass index(BMI)of all inclusive patients.Detection of related biochemical indicators such as blood sugar,blood lipids.Serum FGF19,heme oxygenase-1(HO-1),malondialdehyde(MDA),glutathione peroxidase(GSH-Px)levels were measured by enzyme-linked adsorption immunosorbent assay(ELISA),and superoxide peroxide dismutase(SOD)level was determined by biochemical method.The differences in outcome measures between the two sets were compared.The correlations between FGF19 level and HO-1,SOD,MDA,GSH-Px,CIMT and brachial ankle pulse wave velocity(baPWV)were analyzed by Pearson correlation analysis.Multiple linear regression analysis was performed to analyze the influencing factors of FGF19.Results:Compared with the control group,CIMT,baPWV,HO-1,MDA increased in the experimental group(P<0.01),FGF19,SOD and GSH-Px decreased significantly compared with the control group(P<0.01).Correlation analysis showed that the serum FGF19 level of the experimental group was negatively correlated with HO-1,MDA,CIM,baPWV(r=-0.668、-0.657、-0.901、-0.734,P<0.01)were positively correlated with SOD and GSH-px(r=0.345、0.760,P<0.01).Multiple linear regression analysis showed that FGF19,HO-1,SOD,MDA,GSH-PX were all the possible influencing factors of CIMT(P<0.05).Conclusion:Serum FGF19 level in T2DM patients has a certain relationship with oxidative stress,and has important clinical significance in predicting the occurrence and progression of AS.