HR-HPV阳性宫颈癌血浆中miRNA-10b、miRNA-664、FEZF1-AS1的表达及其对预后的评估价值

Expression of miRNA-10b,miRNA-664,FEZF1-AS1 in Plasma of HR-HPV-positive Cervical Cancer and Its Appraisal Value on Prognosis

目的 探究高危型人乳头瘤病毒(HR-HPV)阳性宫颈癌患者血浆中微小RNA-10b(miR-10b)、微小RNA-664(miR-664)、FEZ家族的锌指蛋白1反义RNA1(FEZF1-AS1)的表达水平及其对预后的评估价值。方法 选取HR-HPV阳性宫颈癌患者49例作为肿瘤组,另选HR-HPV阳性子宫良性病变患者49例作为对照组,检测2组血浆miR-10b、miR-664、FEZF1-AS1表达,并分析其与患者临床病理、治疗效果及预后关系。结果 宫颈癌组miR-10b、miR-664、FEZF1-AS1均高于对照组,差异明显(P<0.05)。血浆miR-10b、FEZF1-AS1、miR-664的表达情况和宫颈癌淋巴结转移、临床分期、病理分级以及分化程度存在一定的相关性(P<0.05)。治疗后miR-10b、FEZF1-AS1、miR-664浓度水平均出现下降,其中相较于治疗无效患者,miR-10b、FEZF1-AS1、miR-664水平在治疗有效的患者中更低,差异明显(P<0.05)。生存曲线显示,miR-10b、miR-664、FEZF1-AS1高表达患者2年生存率、无进展的生存期(PFS)和总体生存时间(OS)均低于其低表达患者,差异明显(P<0.05);Pearson相关性分析显示,miRNA-10b、miRNA-664与FEZF1-AS1表达为正相关关系(γ=0.321、0.293,P<0.05)。结论 在HR-HPV阳性宫颈癌患者的血浆内miR-10b、FEZF1-AS1、miR-664表达的水平升高更为明显,且与患者临床病理方面存在一定的相关性,可用于宫颈癌诊断和预后评估。

Objective To investigate the expression levels of microRNA-10b(miR-10b),microRNA-664(miR-664)and zinc finger protein 1 antisense RNA1(FEZF1-AS1)of FEZ family in the plasma of high-risk human papillomavirus(HR-HPV)-positive cervical cancer patients and their prognostic value.Methods 49 patients with HR-HPV positive cervical carcinoma were selected as the tumor group,and another 49 patients with HR-HPV positive benign uterine lesions were selected as the control group.The plasma expression of miR-10b,miR-664 and FEZF1-AS1 was detected in the 2 groups,and its relationship with clinical pathology,therapeutic effect and prognosis was analyzed.Results miR-10b,miR-664 and FEZF1-AS1 in cervical cancer group were significantly higher than those in the control group(P<0.05).There was a certain correlation between the expression of plasma miR-10b,FEZF1-AS1,and miR-664 and lymph node metastasis,clinical stage,pathological grade,and differentiation in cervical cancer(P<0.05).After treatment,the concentrations of miR-10b,FEZF1-AS1,and miR-664 decreased,and the levels of miR-10b,FEZF1-AS1,and miR-664 were lower in patients who responded to treatment compared with patients who did not respond to treatment,and the differences were significant(P<0.05);the survival curves showed that the 2-year survival rate,progression-free survival(PFS),and overall survival time(OS)of patients with high miR-10b,miR-664,and FEZF1-AS1 expression were lower than those of patients with low expression,and the differences were significant(P<0.05);Pearson correlation analysis showed that miRNA-10b,miRNA-664,and FEZF1-AS1 expression were positively correlated(γ=0.321,0.293,P<0.05).Conclusion The expression levels of miR-10b,FEZF1-AS1 and miR-664 in the plasma of patients with HR-HPV positive cervical cancer are more significantly increased,and there is a certain correlation with the clinicopathology of patients,which can be used for the diagnosis and prognosis evaluation of cervical cancer.