小麦-条锈菌互作过程中TaRLK3D.2功能初步研究

Preliminary study of the TaRLK3D.2 function in the interaction between wheat and Puccinia striiformis f.sp.tritici

小麦条锈病是由小麦条锈菌引起的气传性真菌病害,在全球小麦种植区广泛发生,严重威胁小麦的安全生产。充分利用寄主抗病性是防治条锈病最经济的方法。富含亮氨酸重复类受体蛋白激酶(leucine-rich repeat receptor-like protein kinase,LRR-RLK)作为RLK家族最大分支的模式识别受体,在防御病菌入侵方面发挥重要作用。本研究系统鉴定了小麦LRR-RLKs家族成员,从小麦与条锈菌互作的转录组数据库中筛选出了43个显著差异表达的LRR-RLKs,最后选取了在亲和与非亲和互作过程中都显著上调表达的TaRLK3D.2为对象,利用实时定量PCR、亚细胞定位及大麦条纹花叶病毒介导的基因沉默初步研究了该基因在小麦与条锈菌互作过程中的功能。结果表明TaRLK3D.2定位于植物细胞膜,瞬时沉默TaRLK3.2后,条锈菌侵染严重度显著下降,生长发育变慢。作为典型的LRR-RLKs家族成员,TaRLK3D.2在小麦条锈菌侵染过程中可能行使感病功能。本研究初步明确了该基因的功能,以该基因为靶标通过基因编辑或诱变获取稳定的功能缺失突变体可创制持久抗条锈病材料,为生产服务。

Wheat stripe rust is an airborne fungal disease that occurs in all the wheat-growing areas worldwide and seriously threatens the safety of wheat production.Deploying the resistance cultivar is the most effective method to control this disease.Leucine-rich repeat receptor-like protein kinase(LRR-RLK),as the pattern recognition receptor of the largest subfamily of RLK,plays an essential role in preventing pathogen invasion.The present study systematically identified the wheat LRR-RLK family members and screened 43 significantly differentially expressed LRR RLKs from the transcriptome data in the competable manner of wheat vs Pst.Finally,TaRLK3D.2,which was significantly up-regulated both in compatible and incompatible interaction,was selected for functional analysis in the interaction processing through the real-time qRT-PCR,the subcelluar localization of its encoding protein,and barley stripe mosic virus-mediated silencing technology.Our results showed that the TaRLK3D.2 was located in the plant cell membrane.Based on the transiently silencing induced by barley stripe mosaic virus,TaRLK3.2 knocked down plant inhibited the growth of Pst resulting the significantly reduced disease severity,indicating that TaRLK3D.2 encoding a typical LRR-RLK and might act as a susceptibility gene in the process of wheat stripe rust fungous infection.Our preliminary study explored the function of TaRLK3D.2 gene that could be as a potential target used in resistance breeding of the durable stripe rust resistant material created through stable gene editing or mutation.