引起我国葡萄黑根病的Dactylonectria novozelandica的鉴定

Identification of Dactylonectria novozelandica causing black foot disease of grapevine in China

葡萄黑根病(Black foot disease)是重要的葡萄枝干病害之一,全球葡萄主要种植区均有发生[1],近两年我国有该病害发生的报道[1-2]。田间,黑根病主要危害8年树龄以下的幼树,症状主要表现为发芽异常,枝条节间缩短,叶片失绿黄化,根部出现褐色至黑色坏死等[3-5]。引起葡萄黑根病的病原菌复杂多样,已报道8个属34种病原菌[1]。

Black foot disease is one of the most important grapevine trunk diseases in the world.In this study,three samples from diseased grapevine trunks were collected from three vineyards in Beijing municipality and Hebei province.The potential pathogen was isolated,purified,and identified.In addition,pathogenicity of the isolates was tested to fulfill Koch′s postulates,and the effect of temperature on their mycelial growth was investigated.According to morphological characteristics and a multi-gene phylogenetic analysis with sequences of ITS,EF 1-α,HIS3,and TUB2,five isolates were identified as Dactylonectria novozelandica,which was the causal agent of grapevine black foot disease.Moreover,the pathogen was able to grow between 10 and 30℃.The optimum temperature for mycelial growth was 25℃.This is the first report of grapevine black foot disease caused by D.novozelandica in China.