山东省葡萄白腐病病原菌鉴定

Identification of the pathogen Coniella vitis causing grape white rot in Shandong Province

葡萄白腐病是世界范围内葡萄种植区的重要病害,每年可导致葡萄减产20%~30%[1-2],该病的病原菌有Coniella diplodiella (Speg.) Petrak & Sydow、C. fragariae (Oudem) B. Sutton、Pilidiella castaneicola和C. vitis[3-7]。1999年,LIU等[1]通过形态学研究明确了我国白腐病的病原为C. dip-lodiella。

From 2019 to 2021,grape white rot was reported in Shandong Province.In this study,we performed the isolation from the diseased samples from five different regions,and fifty fungal strains were obtained.The further analyses for 25 strains out of 50 based on morphological observation and multi-locus sequence analyses of ITS,EF-1α,and LSU were conducted and all identified as Coniella vitis.The pathogenicity tests on detached grape leaves,fruits,or living plants for five representatives from the 25 strains exhibited the typical symptoms of the disease,which were similar with that in the field conditions.To the best of our knowledge,it was the first time that C.vitis was identified to be the pathogen of grape white rot disease in Shandong Province,which will provide a scientific basis for the diagnosis and control.