枸杞马铃薯纺锤块茎类病毒的鉴定

Identification of potato spindle tuber viroid in Lycium Linn

0 引言枸杞(Lycium Linn.)属于茄科枸杞属落叶灌木,为药食兼用植物,具较高的药用和食用价值,是我国传统中药材和重要的经济作物[1]。随着枸杞产业的快速发展,需求量逐年增加,人们对枸杞品质的要求也越来越高。病毒是植物常见病原物的一种,病毒感染寄主后会利用寄主成分进行复制,通常会引起寄主生长缓慢、树势衰退、果实品质下降等问题。

Potato spindle tuber viroid(PSTVd)is a kind of viroid with great harm.In this study,high-throughput sequencing combined with sanger sequencing were used to identify possible viruses in Lycium Linn.samples,and PSTVd was identified in Lycium Linn.for the first time.Six pairs of primers were designed,and suitable primers were screened for RT-PCR detection of PSTVd.RT-PCR was carried out on 38 different varieties of Lycium Linn.samples,and the results showed that the detection rate for PSTVd was 52.6%(20/38).The complete genome sequences of 19 PSTVd isolates were obtained by cloning and sequencing.The sequence analysis showed that the nucleotide homology between the sequences was 96.1%-100%.The PSTVd Lycium Linn.isolates and the reported potato and tomato isolates were subjected to phylogenetic tree analysis,and the PSTVd isolates were divided into three groups,two of which only contained Lycium Linn.isolates.This study enriches the host range of PSTVd,and also provides a basis for future research on the pathogenicity of Lycium Linn.PSTVd.