棉花GDP-甘露糖焦磷酸化酶基因(GMP)家族的鉴定及分析

Identification and analysis of the GDP-mannose pyrophosphorylase gene(GMP)family in Gossypium hirsutum L.

GDP-甘露糖焦磷酸化酶(GDP-mannose pyrophosphorylase,GMP)是植物多糖合成途径中的关键酶。本文对陆地棉(Gossypium hirsutum L.)GMP基因家族成员进行了鉴定,分析了其在不同组织及非生物胁迫下的表达模式,并与其他模式植物进行了物种间的进化分析。结果显示,陆地棉和海岛棉(G.barbadense L.)均包含38个GMP家族成员,雷蒙德氏棉(G.raimondii Ulbrich)包含19个成员,亚洲棉(G.arboreum L.)包含18个成员。系统发育分析将不同植物的141个GMP分成了6个亚组,同一亚族GhGMP成员具有相似的基因结构和保守基序,且启动子区含有大量的激素应答、植物生长发育以及胁迫响应相关的顺式作用元件。转录组数据分析结果表明,GhGMPs基因在叶片中的表达量最高,非生物胁迫后,部分基因的表达显著上调。

GDP-mannose pyrophosphorylase(GMP)is a key enzyme in the plant polysaccharide synthesis pathway.In this paper,members of the GMP gene family in Gossypium hirsutum L.were identified,their expression patterns in different tissues and under different abiotic stresses were analyzed,and evolutionary analysis between species and GMP gene families of other model plants was carried out.Results showed that G.hirsutum L.and G.barbadense L.both contained 38 GMP family members,G.raimondii Ulbrich contained 19 members,and G.arboreum L.contained 18 members.Based on phylogenetic analysis,141 GMPs from different plants were divided into six subgroups.GhGMP members of the same subfamily exhibited similar gene structures and conserved motifs,and the promoter region contained many cis-acting element related to hormone response,plant growth and development,and stress response.Transcriptomic analysis showed that the expression of GhGMP genes was highest in the leaves,and some genes were significantly up-regulated after abiotic stress.