摘要
采用生物信息学方法,从甘蓝型油菜(Brassica napus L.)基因组数据库中筛选鉴定MAP70s基因家族成员,对鉴定得到的BnaMAP70s基因家族成员的序列特征进行生物信息学分析,同时利用qRT-PCR方法分析该基因家族成员在不耐渍和强耐渍甘蓝型油菜品种幼苗中的基因表达水平。结果显示,本研究共鉴定得到19个BnaMAP70基因家族成员,分布在11条染色体上,可分为5个亚家族。BnaMAP70s启动子的上游存在厌氧胁迫、响应植物激素等相关元件,表明BnaMAP70s可能参与植株生长发育和渍水胁迫调控;转录水平存在品种特异性、植株部位特异性、时间特异性。q RT-PCR分析结果表明,BnaMAP70-1和BnaMAP70-4在渍水胁迫下出现表达差异,说明这两个基因受渍水胁迫的调控。
MAP70 family members were screened and identified using bioinformatics based on the genomic database of Brassica napus L.,with bioinformatics analysis performed on the sequence characteristics of the identified BnaMAP70 gene family members.Expression levels of the BnaMAP70 genes were analyzed using qRT-PCR in seedling varieties with waterlogging sensitivity and tolerance.In total,19 members of the BnaMAP70 gene family were identified,distributed on 11 chromosomes and divided into five subfamilies.The upstream elements involved in anaerobic stress and phytohormones of the BnaMAP70 promoter indicated that they may be involved in plant growth,development,and regulation of waterlogging stress.The transcription levels of genes were species-specific,plant site-specific,and time-specific.The qRT-PCR results showed that BnaMAP70-1 and BnaMAP70-4 were differentially expressed under waterlogging stress,indicating that these two genes may be regulated by waterlogging stress.
作者
黄郢
翟璐
谢伶俐
徐劲松
张学昆
许本波
Huang Ying;Zhai Lu;Xie Ling-Li;Xu Jin-Song;Zhang Xue-Kun;Xu Ben-Bo(Engineering Research Center of Ecology and Agricultural Use of Wetland,Ministry of Education,Yangtze University,Jingzhou,Hubei 434025,China;College of Agriculture,Yangtze University,Jingzhou,Hubei 434025,China)
出处
《植物科学学报》
CAS
CSCD
北大核心
2023年第5期647-656,共10页
Plant Science Journal
基金
国家重点研发计划项目(2017YFD0101703)
湖北省农业厅项目(鄂农油[2021]9号)。