超疏水性三维银纳米树SERS基底的制备及用于阿奇霉素检测

Preparation of superhydrophobic 3D silver nanotree SERS substrates for the detection of azithromycin

制备了一种超疏水性三维银纳米树表面增强拉曼散射(SERS)基底,利用扫描电子显微镜(SEM)、能量色散X射线光谱(EDS)及X射线衍射(XRD)对三维银纳米树进行表征,利用接触角(CA)分析其疏水性能。以罗丹明B(RB)为拉曼探针进行条件优化,并考察阿奇霉素(AZM)在该基底上的SERS性能,发现该SERS基底对AZM具有较强的拉曼增强效应,在1.0×10^(-11)~1.0×10^(-9)mol/L范围内,AZM浓度的对数值与拉曼峰强度I_(SERS)呈良好的线性关系,线性拟合方程为I_(SERS)=4098logc+47769(R^(2)=0.987),检出限为4 pmol/L。使用该方法检测AZM分散片、人血清及猪血清中的AZM,加标回收率在91.2%~118.1%之间,相对标准偏差为1.1%~6.2%。

A superhydrophobic 3D silver nanotree surface enhanced Raman spectroscopy(SERS)substrate was synthesized,and characterized by scanning electron microscopy(SEM),energy dispersive spectrometer(EDS)and X-ray powder diffraction(XRD).Its hydrophobic properties were analyzed by contact angle(CA).Rhodamine B(RB)was used as Raman probe to optimize the conditions.The SERS properties of azithromycin(AZM)on this substrate were investigated,showing that the SERS substrate had a high Raman enhancement effect on AZM.The I_(SERS)presented a good linear relationship with the logarithm concentration of AZM as I_(SERS)=4098logc+47769(R^(2)=0.987)in the range of 1.0×10^(-11)-1.0×10^(-9)mol/L,and the detection limit was 4 pmol/L.This method was used to analyze AZM content in AZM dispersible tablets,human serum and porcine serum,with the spiked recoveries in the range of 91.2%-118.1% and relative standard deviations of 1.1%-6.2%.