Minitab软件在优化氟氢可的松检测方法中的应用

Minitab Software in Fludrocortisone Detection Method Application of Optimization

应用Minitab软件,优化液相色谱-串联质谱测定牛乳中氟氢可的松方法的参数条件。利用Minitab软件中单因素方差分析、实验设计(Design of experiment,DOE)等工具,研究称样量、提取试剂、复溶试剂、洗脱试剂对回收率的影响,确定最优的检测条件。结果表明,优化参数条件后,添加水平分别为0.4、0.8、4μg/kg时,回收率达到72.3%~109.3%,相对标准偏差达到6.7%~12.0%,满足方法学验证要求。通过应用Minitab软件,可以准确找到影响检测结果的关键影响因子及最佳参数条件,为检测方法优化提供科学依据,从而保证检测结果更加准确、可靠。

Minitab software was used to optimize the parameter conditions of determination of fludrocortisone in milk by liquid chromatography-tandem mass spectrometry.Using Minitab software,one-way ANOVA and DOE experimental design were carried out.The effects of sample weighing,extraction reagent,redissolution reagent and elution reagent on recovery rate were studied,and the optimal detection conditions were determined.The results showed that after optimizing the parameter conditions,when the addition levels were 0.4,0.8 and 4μg/kg,the recovery rate reached 72.3%~109.3%,and the relative standard deviation reached 6.7%~12.0%,which met the requirements of methodology verification.By using Minitab software,we can accurately find the key influencing factors and the best parameter conditions that affect the detection results,and provide scientific basis for the optimization of detection methods,so as to ensure that the detection results are more accurate and reliable.